The Xiaoxianxiong Decoction Of The Experimental Treatment Of Phlegm-heat Obstructing Lung-acquired Pneumonia

Objective:rat Endotracheal intubation instillation of E. coli endotoxinphlegm-heat obstructing lung-acquired pneumonia model, observed changesin the lung tissue of rats, and tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ), interleukin-2receptor (IL-2R), interleukin-8(IL-8) changes, so thatthe experimental method to investigate the heat phlegm chest caking dispersedprescription Xiaoxianxiong Decoction phlegm-heat obstructing lung-acquiredpneumonia and its mechanism to provide experimental basis for clinicalapplication.Pneumonia (pneumonia) is explained as the terminal airways, alveolarand interstitial lung inflammatory disease. Pneumonia is closely relatedpathogens and host factors. If the pathogen invasion and (or) the host’simmune defense system functionality is limited, that cause pneumonia.Pathogens can cause pneumonia: by the following ways:aerosol pathway;hematogenous spread; close to the spread of the infection; respiratory tractcolonization aspiration. The breeding of pathogens cause alveolar capillarycongestion, edema, inflammatory cell infiltration, and fibrin exudation.Bacterial pneumonia is a common type of pneumonia, the most commoninfection caused by pathogenic microorganisms. Period of not widely usedantibacterial drugs, bacterial pneumonia crowd caused a great deal of healththreats, the use of antimicrobial agents pole the pneumonia mortalitydecreased significantly. With the enhancement of the ability of theanti-drug-resistant pathogens the mutation causing pneumonia total mortalityand even to rebound upward trend.Bacterial pneumonia in the evolution of the disease and more fever,cough, chest tightness, cough vomiting yellow sputum and other symptoms,like the diseases of the chest phlegm-heat from Shanghan Lun, chest abdominal swelling nausea according to the pain, expectoration of thickyellow greasy yellow tongue coating, slippery pulse number of the samesymptoms of the disease, which is the treatment of Xiaoxianxiong Decoctionphlegm-heat obstructing lung type pneumonia square card syndromefoundation.The experimental application of rat endotracheal intubation instillationof Escherichia coli endotoxin rat phlegm-heat obstructing lung-acquiredpneumonia model, while giving Xiaoxianxiong Decoction western medicineantibiotic control rat tumor necrosis factor-α(TNF-α), interferon-γ (IFN-γ),interleukin-2receptor (IL-2R) and interleukin-8(IL-8) and other indicators ofchange, and to explore the mechanism of action of the XiaoxianxiongDecoction for the treatment of rats phlegm-heat obstructing lung pneumoniawhich can provide a theoretical basis for better clinical application.Method:1A selection of healthy and clean40female Wistar rats, body weight250±10g, adaptive feeding a week, after measuring body temperature, one by oneweighing label by weight, in accordance with the principle of randomallocation is divided into5groups of8: normal group (Normal control groupN), model group (Model group, M), Xiaoxianxiong Decoction group(Xiaoxianxiong Decoction group, X), the Western medicine antibiotic group(Antibiotic group, A), Xiaoxianxiong Decoction and Western medicine for theantibiotic group (Xiaoxianxiong Decoction and Antibiotic group, XA). Exceptthe blank group, the other groups are used endotracheal intubation instillationof Escherichia coli endotoxin production of phlegm-heat obstructinglung-acquired pneumonia rat model.2experimental drug delivery: According to the the human dosageexperimental animals dose conversion formula, calculate the dosage in rats.The five groups were fed with normal diet, N, M group was given distilledwater (10ml·kg-1·d-1); the remaining X group, A group, and XA group aregiven (11.7g·kg-1·d-1) Xiaoxianxiong Decoction,(9mg·kg-1·d-1) antibiotics,Xiaoxianxiong Decoction and antibiotics by gavage. Every day treats2times, treats continuously for3days.3detection of indicators: the end of the experiment to the start of theexperiment to the end of the morning and evening measure the bodytemperature of rats2times normal drinking eating; femoral artery blooddetected in the blood of rats interferon-γ (IFN-γ), interleukin-2receptor(IL-2R), and interleukin-8(IL-8) changes in the indicators; take the right partof the lung tissue quickly placed fixed in4%paraformaldehyde, dehydrated,embedded in paraffin, HE staining, for light microscope lung structure andchange, and detect the expression of tumor necrosis factor-α(TNF-α).Results:1A general and rat blood test resultsBlank (normal control) rats mental condition, body temperature, hair,activities, diet, drinking water did not change significantly; model ratsincreased body temperature up to39.5°C, hair messy bristling, coat glossymissing, eating drinking water in poor condition.In the cages they curled up atthe corner, scrambling irritability, have the apparent respite action and breathsounds severe symptoms see rat limbs cyanosis, difficulty in breathing; drawnone day before, the model group, the most severe symptoms, some rats weightloss; Xiaoxianxiong antibiotic group and Xiaoxianxiong Decoction rats diet,body temperature, and the activities of the overall state of the antibiotic groupcompared with the model group had varying degrees of improvement. Thetemperature there is a significant difference (P<0.01) in the blank with themodel group, as well as among the treatment groups. The body temperature ofN group and M group rats after modeling were no difference (P>0.05), Xgroup, A group, XA group, changes in body temperature differences weresignificant (all P<0.01).The rat blood routine measurement results, compared with the controlgroup, the neutrophils (PMN) white blood cell (WBC) of model group weresignificantly higher (P<0.01). Xiaoxianxiong Decoction group, antibioticgroup, WBC level of Xiaoxianxiong Decoction antibiotic group comparedwith the model group decreased a significant difference (P<0.01). 2rats blood index test resultsIL-8measurement results, compared with the control group rats, the IL-8levels were significantly increased (P<0.01). Xiaoxianxiong Decoction groupcompared with the model group, antibiotic group, Xiaoxianxiong Decoctionantibiotic rats IL-8levels had a significant difference (P<0.01). Comparedwith the antibiotic group Xiaoxianxiong Decoction antibiotics rats IL-8levelsdecreased significant differences (P<0.01).IL-2R measurement results, compared with the control group, model group,IL-2R levels were significantly increased (P<0.01). Xiaoxianxiong Decoctiongroup compared with the model group, antibiotic group, XiaoxianxiongDecoction antibiotic group IL-2R levels had a significant difference (all P<0.01). Antibiotic group IL-2R levels decreased compared with the antibioticgroup, Xiaoxianxiong Decoction group had significant differences (P<0.01).IFN-γ measurement results, compared with the control group, the modelgroup, IFN-γ levels were significantly increased (P<0.01). XiaoxianxiongDecoction group compared with the model group, the antibiotic group, thelevels of IFN-γ trapped Xiaoxianxiong Decoction antibiotic group, significantdifferences (all P<0.01). Compared with the antibiotic group XiaoxianxiongDecoction antibiotic group IFN-γ levels there are significant differences (P<0.01).3light microscope lung tissue pathological morphologyNo pathological changes in the lung tissue of the control group; model group,HE staining red blood cell leakage, visible to a large number of inflammatorycell infiltration, and alveolar slurry filled, the part of the alveolar wallthickening; other groups, HE staining of lung tissue pathological changescompared with the model group was significantly improved, the alveolar lessatrophy, lung lower lobe bronchial lumen mild expansion alveolar wallthickening lighter and a small amount of inflammatory cell infiltration andexudation.4the expression of Tumor Necrosis Factor-α (TNF-α).TNF-α measurement results with the control group compared with the model group mainly in the alveolar and bronchial wall expression, part of the lungtissue also have scattered in the expression; Xiaoxianxiong Decoction group,antibiotic group, Xiaoxianxiong Decoction and antibiotic group comparedwith the model group significantly reduce (all P<0.01), with the antibioticgroup compared with the Xiaoxianxiong Decoction antibiotic group decreaseddifferences (P<0.05).Conclusion:1Xiaoxianxiong Decoction can reduce rat tumor necrosis factor (ofTNF-α), interferon-γ (of IFN-γ), interleukin-2receptor (IL-2R), andinterleukin-8(IL-8) level of bacterial resistance pneumonia treatment effect.2to promote mature T-lymphocyte transformation and generate, improvecellular immunity, reduce inflammation, reduce secretions generation, mightbe an effective mechanism of Xiaoxianxiong Decoction.3Xiaoxianxiong Decoction composed of active ingredients of drugs haveinhibitory effects on specific bacteria, to promote the release of a variety ofcytokines, the body repair disease damage faster, better disease prognosis.Meanwhile, adjusts the reassignment immunity function effectively, causes theorganism anti-infection ability enhancement, which is an advantage for Xiaoxianxiong Decoction better than Western medicine and antibiotics.