Effect Of Adhesion To Fibronectin Via β1Integrin On Proliferation And Apoptosis In A2780Cells

Objective:Ovarian cancer is the most lethal one of the female reproductive system malignancies because its elevated incidence and high mortality. Some ovarian cancer that have localized outward appearance, always migrates to peritoneum, greater omentum, retroperitoneal lymph node. Besides, the apoptotic resistance against chemotherapy in ovarian cancer cells is also due to the high mortality. The interactions between cell and extracellular matrix in tumor micro-environment have major impact on metastasis and chemical resistance of ovarian cancer.(31integrin, belongs to subgroupβ1of integrins, is cell adhesion factor receptor, which highly express on the ovarian tumor cell surface. β1integrins, when activated by its ligand-FN, influence cell morphology, motility, growth, thus affecting tumor cell’s invasion, metastasis and apoptosis. The research investigate the effects of adhesion to fibronectin via β1integrin on proliferation and apoptosis in A2780cells and make a tentative study of the signal transduction of β1integrin.Methods:Cultured the human ovarian cancer cell line A2780in vitro as the target of the study. To set up three experimental groups which separately deal with poly-lysine, FN and FN+antibody of integrin. The activity of integrin β1was induced by FN—ligand of β1integrin and blocked by the monoclonal antibody of β1integrin. Cell proliferation were measured by CCK-8assay; the apoptosis and cell cycle were analyzed by flow cytometry; real-time PCR was used to detect expression of Aurora B and surviving gene. The data from the experiment was represented by x±s and analyzed by statistics software SPSS17.0. Student’s test was used to analyze between the treatment groups. P<0.05said differences are statistically significant.Results:The OD values in the three experimental groups in36hour,72hour are0.405±0.021、0.623±0.030、0.480±0.019;0.886±0.022、1.139±0.022、0.959±0.027. The poly-lysine group compared with FN group and FN group compared with FN+Anti-β1group, the results at the same time were statistically significant difference(p<0.05). Compared to the Poly-lysine control, the percent of cells in S or G2/M were increased (P<0.05). β1integrin monoclonal antibody can block the activity induced by cell adhesion to FN via integrin β1in A2780(P<0.05). The apoptosis rates of the poly-lysine group, FN group, FN+Anti-β1group were (24.3±1.38)%,(8.82±0.725)%,(20.69±1.128)%. Poly-lysine group as FN group (P=0.006)、FN group as FN+Anti-β1group (P=0.009) were statistically significant different. The expression of Aurora B and Survivin gene were increased (P<0.05).Conclusion:β1integrin mediated FN adhesion can increased Aurora B and Survivin gene levels, and that may be related to cell growth and apoptosis inhibition induced by FN adhesion.