| Objective:Through joint detection EB1 and Aurora-B patients in hospital surgery of normal ovarian tissue,epithelial ovarian carcinoma tissue and epithelial ovarian benign tumors expression in the organization,and respectively discuss EB1,Aurora-B with ovarian epithelial carcinoma clinical staging and differentiation degree of relationship,if there is a correlation analysis EB1 and Aurora-B,and to assess EB1 and Aurora-B prognostic value in patients with epithelial ovarian carcinoma.Methods:1.The object of study: from January 2014 to January 2017 to nanchang university first affiliated hospital of obstetrics and gynecology surgical treatment of epithelial ovarian cancer patients,a total of 80 cases,benign epithelial oarian cancer patients,a total of 20 cases,and other selected health in patients with ovarian tissue(surgical treatment for uterine diseases,subject to consent by the patients,the line of bilateral adnexectomy)in patients with a total of 20 cases.The general clinical data of the patients were collected,and the clinical and pathological data of all the cases were complete.There were no other tumors or severe complications(hypertension,diabetes,other acute and chronic diseases,etc.).2.Tissue collection: collect tissue wax blocks.3.Detection indicators and methods:(1)the expression levels of EB1 and Aurora-B in the three groups were detected by immunohistochemistry.The correlation between EB1 and Aurora-B and its clinical significance were studied.(3)to analyze the relationship between the two factors and the clinicopathological characteristics of ovarian epithelial carcinoma.4.Collected the clinical and pathological data of the patients,followed up the survival of the patients through telephone,WeChat and outpatient review,and calculated the survival curve.5.Statistical analysis: SPSS 22.0 was used to analyze all the research data.Measurement data to ` x±s,group using single factor analysis of variance,the difference between the two compare the LSD-t test,expressed as a percentage of count data,using Χ 2 inspection comparison group,the difference between analysis and prognosis of ovarian cancer by Kaplan Meier-survival analysis,expressed as a P ﹤ 0.05 difference was statistically significant.Results:1.EB1 protein in normal ovarian tissue,benign epithelial tumors,epithelial carcinoma tissue in the positive expression rate of 10%,30% and 88.75%,respectively,in epithelial carcinoma of the positive expression rate is significantly higher than the former two groups,the difference was statistically significant(P < 0.05),and normal ovarian tissue and benign epithelial tumor group,there was no statistically significant difference(P > 0.05).The lower the degree of tumor differentiation and the higher the FIGO stage,the higher the positive rate of EB1 protein.The positive expression rates of EB1 in the high and low age groups were 79.31% and 77.27%,respectively,with no statistically significant difference(P > 0.05).The positive rate of EB1 was 83.33% in patients with lymphatic metastasis and 84.38% in patients without lymphatic metastasis,with no statistically significant difference(P > 0.05).The positive rate of EB1 in serous adenocarcinoma was 82.5%,that in mucinous adenocarcinoma was 73.33%,that in endometrioid carcinoma was 75%,and that in clear cell carcinoma was 72.92%,so there was no significant difference in EB1 expression in different tissue types.The positive expression rate of EB1 in preoperative adjuvant chemotherapy was 69.57%,and that in preoperative non-adjuvant chemotherapy was 68.42%.The positive expression rate of EB1 was 70.83% in platinum-class drugs and 78.57% in platinum-class drugs,showing no significant difference in expression.2.In normal ovarian tissue,benign epithelial tumor tissue and epithelial carcinoma tissues Aurora-B protein positive expression rate of 40%,45% and 70% respectively,the positive expression rate of the preceding two group was obviously lower in epithelial carcinoma group,the difference was statistically significant(P < 0.05),and normal ovarian tissue and benign epithelial tumor group,there was no statistically significant difference(P > 0.05).The lower the degree of tumor differentiation and the higher the FIGO stage,the higher the positive rate of Aurora-B protein.The positive expression rates of Aurora-B in high and low age groups were 60.34% and 63.63%,respectively,with no statistically significant difference(P > 0.05).The positive rate of Aurora-B in tissues was 60.42% in patients with lymphatic metastasis and 59.38% in patients without lymphatic metastasis,with no statistically significant difference(P > 0.05).The positive rate of Aurora-B in serous adenocarcinoma was 62.5%,mucinous adenocarcinoma was 66.67%,endometrioid carcinoma was 66.67%,and clear cell carcinoma was 61.54%,so there was no significant difference in the expression of Aurora-B in different tissue types.The positive expression rate of Aurora-B in preoperative adjuvant chemotherapy was 52.17%,and that in preoperative non-adjuvant chemotherapy was 49.12%.The positive expression rate of Aurora-B in platinum-class drugs was 62.5%,and that in platinum-class drugs was 66.07%.3.Spearman was used to analyze the correlation between EB1 and Aurora-B expression,and the results showed that EB1 and Aurora-B showed a positive correlation in ovarian epithelial carcinoma(r=0.46,P<0.05).4.Kaplan-meier survival curve was used to analyze the correlation between EB1 and Aurora-B expression and disease-free survival rate and overall survival rate of ovarian cancer patients.The results showed that,compared with ovarian cancer patients with negative expression of EB1 and Aurora-B,disease-free survival rate and overall survival rate of the positive expression group were significantly reduced,and the difference was statistically significant.Conclusion:1.The positive expression rates of EB1 and Aurora-B in ovarian epithelial carcinoma were significantly higher than that in normal ovarian tissues and ovarian epithelial benign tumors,suggesting that EB1 and Aurora-B may be involved in the development of ovarian epithelial carcinoma.Its expression is related to the degree of differentiation and FIGO stage.2.The disease-free survival rate and overall survival rate of EB1 and Aurora-B positive expression group were significantly shorter than that of the negative expression group,which has certain guiding significance for the prognosis of ovarian epithelial carcinoma.3.EB1 has a positive correlation with Aurora-B,indicating that the two have a synergistic effect,and the combined detection of the two may improve the accuracy of early diagnosis,and may be used as a potential therapeutic target. |
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