Effect Of Drugs For External Use On The Expression Of TNF-α In The Exercise-induced Muscle Chronie Damage

Objective:Improvement of skeletal muscle capabilities and Athletic Ability has a close relationship,exercise training can cause changes in skeletal muscle of some cytokines, such as tumornecrosis factor (TNF-alpha), chronic injury of this factor in the regulation of skeletal muscleplays an important role. Through the analysis of changes in expression of TNF-alpha inskeletal muscle of chronic injury, explore Qi Xiaotong Plaster on the effect of the treatment ofchronic skeletal muscle injury and possible mechanism of action, provide an objective basisfor better practice.Method:94selected adult male New Zealand rabbits as subjects were randomly divided into thequiet control and experimental groups. The quiet blank control group (n=5); experimentalgroup was divided into four groups:2weeks training group (n=5);3weeks of training anddrug intervention group (n=28), four weeks in training and drug intervention group (n=28), five weeks training and drug intervention group (n=28);3,4,5weeks in each group isdivided into4groups: Tibetan medicine group, help his forest group in the placebo group andblank control group, each group (n=7). High-intensity running and jumping training inaccordance with the training program of experimental animals, each rabbit daily running andjumping about300times, continuous training five days a week, the longest training period offive weeks. Left medial head of gastrocnemius drawn in different sports point in time (weeks),by HE staining observed tissue structural changes, the use of immunohistochemistry visit toFrance detect different time running and jumping sports and drug intervention after eachanimal muscle TNF-alpha expression changes.Results:1. HE staining of skeletal muscle cells:1) the quiet blank control group of muscle cells showing fibrous muscle fibers tightlypacked, obvious stripes, without interruption, nucleus, chromatin, the cytoplasm is evenlydistributed.2)2weeks of training muscle cells cellulose degeneration, degeneration of striatedmuscle cells lead to muscle atrophy or hyperplasia, abnormal nucleus distribution, indicatingthat the muscle degeneration in the repair process or transformation lead to muscle bundle,and some there are still a little vacuoles variability and discoid degeneration.3)3weeks Tibetan medicine group muscle cells no obvious degeneration of musclenuclear evenly distributed;3weeks fibrous Fu Lin group of muscle cells, the stripes isobvious;3weeks the placebo group had very little vacuolar degeneration, nucleus distribution;3weeks in the control group with a small amount of vacuolar degeneration.4)4weeks Tibetan medicine group cytoplasm and muscle nuclear evenly distributedaround the fiber sarcolemma, non-proliferation and swelling or shrinkage, no inflammatorycell infiltration;4weeks the Fu Lin group striated found no variability;4weeks in the placebogroup vacuolar degeneration;4weeks in the control group vacuolar degeneration, nuclearabnormalities, proliferative state.5)5-week Tibetan medicine group overall muscle Nuclear distribution uniform, there isno inflammation, mild increase in a little cell muscle nuclear hypertrophy;5weeks Voltarenlittle vacuolar degeneration, muscle nuclear and chromatin evenly distributed;5weeks theoverall bundle of the placebo group was not denatured, local special treatment increasedmuscle nuclear mild hyperplasia; uniform cytoplasm of five weeks in the control group, asmall amount of inflammatory cell infiltration, the local muscular nuclear proliferation.2drug intervention on exercise-induced muscle the chronic injury TNF-alpha expressionof1) group horizontal comparison results:3weeks group of Tibetan medicine group, helphis forest group and placebo group, respectively, with the control group comparison has asignificant difference, but Tibetan medicine group with the help of his forest group and theplacebo group and two or two comparison not difference sex.4weeks group of Tibetanmedicine group, Fu Lin group and control group compared with the differences in the placebogroup compared with control group does not have a difference, Tibetan medicine group andthe placebo group compared with the difference, Tibetan medicine group Voltaren group doesnot have a difference, the Voltaren group and the placebo group does not have differences.Five weeks group group of Tibetan medicine group and control group compared with thedifferences, Voltaren group, the placebo group compared with control group does not haveany difference, Tibetan medicine group and the placebo group compared with the difference,Tibetan medicine group with Voltaren group less difference, the Voltaren group and theplacebo group does not have differences.2) TNF-alpha expression longitudinal comparison: group of Tibetan medicine3,4,5weeks, three weeks and four weeks compared results with difference,3weeks and5weeksgroup has a significant difference;4weeks significant difference compared with the fiveweeks. Fu Lin3,4,5weeks group comparison,3weeks4weeks compare results havesignificant differences, three weeks and five weeks group comparison also has a significantdifference;4weeks and5weeks group comparison the same has significant differences sex.Placebo3,4,5weeks group, three weeks and four weeks compared results with difference3weeks and5weeks group has a significant difference;4weeks and5weeks compared withthe difference. Conclusion:1.by observing two weeks training control group HE staining and immunohistochemistrysituation shows that chronic exercise muscle damage modeling is very successful, long gamesexacerbate TNF-α expression.2. Qi Xiaotong Plaster and other drug comparison, can effectively inhibit theexercise-induced muscle injury in chronic tumor necrosis factor TNF-α sub overexpressionprevents cell damage intensifies, said minch Xiaotong Plaster chronic injury on skeletalmuscle has a very good intervention.