Effect Of Alpha-melanocyte On The Expressions Of Cutin Cell Growth Factor Receptor And Alpha Sodium Channel Protein In Rat Models Of Renal Ischemia-reperfusion Injury

Objective To study the m RNA and protein expression of(KGFR) and(α-ENa C) as well as theα-MSH in renal and lung tissue in rats with acute ischemial reperfusion(IR) kidney injury.Methods a total of 30 healthy male s-d rats(210- 230g) were randomly divided into 3 equal groups:control group、IR group and α-MSH group. rats of each group were sacrificed at 24 h after modeling.Kidney and lung tissue was harwested and the ratio of wet and dry weight(W/D) of Kidney and lung were calculated and the pathological changes of the tissues were observed under microscope. The protein and m RNA expression of KGFR and α-ENa C in renal and lung tissue were determined with immunohistochemistry and RT-PCR,respectively.Result Compared with control group, W/D of kidney and lung increased significantly in rats of IR group and a-MSH group, while the levels of KGFR and α-ENa C of kidney and lung in rats were lower(P<0.05); Compared with IR group, W/D of kidney and lung in rats of a-MSH group decreased significantly,while the levels of KGFR and α-ENa C of kidney and lung increased gradually(p<0.05),Expression of KGFR and α-ENa C were positively correlated with the W/D of Kidney and lung(p<0.05).Conclusions After renal IR injury, low KGFR and α-ENa C expression was consistent to the kidney and lung injury, α-MSH could increase the protein and m RNA expression of KGFR and α-ENa C in kidney and lung of rats, which probably is one of the mechanisms that reduce the kidney and lung injury.