Study On The Relationship Between Apolipoprotein E Gene Polymorphism And Cerebral Infarction

Objective： To explore the relationship between ApoEpolymorphism and cerebral infarction.Methods：This study was to select200cases of Hospitalizedpatients with cerebral infarction in Second Clinical MedicalCollege neurology of Jilin University in2010December to2012October,Male121cases, female79cases.The selected patientswere diagnosed as cerebral infarction by head CT or MRI. AndConforms to the diagnosis standard of The Fourth AcademicConference on cerebrovascular disease At the same time, select100cases of health examination in the medical examinationcenter as control group,Male57cases,female43cases, All thehealth examinations were excluded diabetes, cerebrovasculardisease, liver and kidney disease, heart disease etc.takeVenous blood of the Patients and controls. Using DNA ExtractionKit extract DNA from white blood cells. Using PCR analysis theDNA. Reaction system is: DNA2μ L,2×Buffer10μ L, DNApolymerase0.4μ L, The upstream and downstream primerrespectively6pmol, Sterile double distilled water6.4μL. Thereaction conditions is: Preheat denatured at95℃for5min, denaturation60s at95℃,Annealing at62℃45s, extend60s at72℃, A total of30cycles,at last extend7min at72℃.Results of PCR amplification products were electrophoresed in2%agarose gel to detecte amplified results. PCR products byHhaI enzyme Cut2hours in37℃water,The digested productsthrough8%polyacrylamide gel electrophoresis observations.Results：The result for each genotype is: ε2/2genotypesshow91bp,83bp and38bp fragment length, ε2/3genotypesshow91bp,83bp,48bp和38bp fragment length, ε2/4genotypesshow91bp,83bp,72bp和38bp fragment length, ε3/3genotypesshow91bp,48bp和38bp fragment length, ε4/3genotypes show91bp,72bp,48bp和38bp fragment length, ε4/4genotypes show72bp,48bp和38bp fragment length. The digested products bypolyacrylamide gel electrophoresis, Cerebral infarction groupof each genotype and allele frequency for: ε2/20.5%、ε2/36%、ε2/47%、ε3/368%、ε3/411%、ε4/47.5%、ε27%、ε376.5%、ε416.5%；The control group of each genotype and allelefrequency for: ε2/22%、ε2/37%、ε2/43%、ε3/379%、ε3/45%、ε4/44%ε27%、ε385%、ε48%. theε3/3genotype is thehighest frequency in the Two groups, And the control group wassignificantly higher than that of the cerebral infarction group（P<0.05）；Theε3allele frequency is the highest, And cerebral infarction group was significantly higher than that of thecontrol group（P<0.05）；the ε4/4andε3/4genotype frequencyis lower than ε3/3genotype in the Cerebral infarction group,And higher than that of the control group,but P>0.05. Thefrequency ofε2/3genotype in cerebral infarction group waslower than the control group, but P>0.05. The frequency of ε2/4genotype in cerebral infarction group and The frequency ofε2allele frequency in Cerebral infarction group were lowerthan the control group, but P>0.05.Conclusion：1. The ε3/3genotype andε3allele may haveprotective effect on cerebral infarction.2. The ε4allelemight be a risk factor for cerebral infarction.