Proteomics Study Of Cerebrospinal Fluid In Multiple Sclerosis Patients

Multiple sclerosis (MS) is a common autoimmune disease with the feature of centralnervous system demyelination. The clinical manifestations of MS are recurrent attacks andrelapsing-remitting. The pathological characteristics include inflammatory infiltrationsurrounding the small vessels, demyelination of myelin sheath, astrocyte proliferation,oligodendrocyte and axon damage. However, the etiology and pathogenesis of MS is knownunclearly. In order to explore the underlying molecular mechanism of MS, specificbiomarker protein related with MS development should be identified firstly. Then, themechanism of MS development and self-remyelination may be studied.Objective: Proteomics analysis was used to inquire differential proteins in CSF of MSpatients in order to detect the disease-specific proteins (DSPs). The purpose of this study isto provide new research evidences for MS clinical diagnosis, treatment, prognosisimprovement, and detectetion of drug targets.Methods:15patients were divided into2groups, MS group and Control group.Two-dimensional gel electrophoresis (2D-DIGE) combined with matrix-assisted laserdesorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used toidentify DSPs in MS patients’ CSF.Results: Compared with Control group,7differtial proteins were identified in MSgroup.(1) Apolipoprotein A-I (ApoA-I) expression up-regulated obviously in1.83timeswith significant statistical difference (P<0.005);(2) Apolipoprotein E (ApoE) expressiondown-regulated obviously in2.37times with significant statistical difference (P<0.005);(3)Beta amyloid precursor protein (β-APP) expression down-regulated obviously in1.91times with significant statistical difference (P<0.005);(4) Clusterin (CLU) expressiondown-regulated obviously in2.16times with significant statistical difference (P<0.005);(5)Transthyretin (TTR) expression down-regulated obviously in1.79times with significantstatistical difference (P<0.005);(6) Fibulin-1(FBLN-1) expression down-regulatedobviously in1.5times with significant statistical difference (P<0.005);(7) Nidogen-2 (NID-2) expression down-regulated obviously in1.72times with significant statisticaldifference (P<0.005).Conclusion:1.7DSPs were identified in MS group, including up-regulated ApoA-Iand down-regulated ApoE, CLU, β-APP&FBLN-1. These DSPs were supposed toparticipant in MS pathogenesis through specific mechanisms.2. It is the first time thatFBLN-1and NID-2was reported down-regulating in CSF of MS patients．Their definitefunctions are unknown and need further studies.