Cerebrospinal Fluid Proteomics Study In GBS Patients With Different Subtypes

GBS (Guillain-Barré syndrome) is an acute inflammatory demyelinating disease ofperipheral nervous system, with pathological changes of lymphocyte and macrophageinfiltration surrounding the small vessels and demyelination of myelin sheath. Few severepatients may be combined with axonal damage. However, etiology of GBS is unknown.Objective: GBS is typically classified into two major subtypes: acute inflammatorydemyelinating neuropathy (AIDP) and acute motor axonal neuropathy (AMAN), but theyhave big difference in etiology, clinical manifestation and prognosis. This study tried toidentify possible disease specific proteins (DSPs) in GBS patients with different subtypesby proteomics study on CSF for first time. The purpose of this study is to explore evidencefor better understanding of pathogenesis, prognosis and treatment biomarker.Methods:20patients were divided into3groups, AIDP group, AMAN groups andControl group. Proteomics analysis of CSF on GBS patients with different subtypes wasmade by two-dimensional difference in gel electrophoresis (2-D DIGE) combined withmatrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOFMS).Results:1. Compared with Control group,4DSPs were identified in AIDP groupincluding ApoD up-regulated3.19bolds, PEDF down-regulated2.76bolds, Cathepsindown-regulated1.70bolds, ApoE down-regulated2.21bolds.2. Compared with Controlgroup,8DSPs were identified in AMAN group including ApoD up-regulated4.15bolds,APP up-regulated1.73bolds, HP up-regualted2.86bolds, PEDF down-regulated3.27bolds,Cathepsin down-regulated1.81bolds, ApoE down-regulated2.61bolds, FADdown-regulated2.82bolds, and TGAb down-regulated2.96bolds.3.4DSPs were identifiedboth in AIDP&AMAN group, including up-regulated ApoD and down-regulated ApoE,Cathepsin,&PEDF.Conclusion:1.4DSPs were identified in AIDP group as up-regulated ApoD anddown-regulated PEDF, Cathepsin&ApoE.2.8DSPs were identified in AMAN group asup-regulated ApoD, APP,&HP, and down-regulated PEDF, Cathepsin, ApoE, FAD,&TGAb.3. For first time, APP was reported up-regulating in CSF of GBS AMAN group, indicating APP possibly involved in GBS development and progress.4.4DSPs wereidentified both in AIDP&AMAN group, including ApoD, ApoE, Cathepsin, and PEDFwith similar regulating changes.