The Value Of IP-10、ApoA-1、ApoB And CD64 In Early Diagnosis Of KD

Kawasaki Disease(KD)is an acute multisystem inflammatory vasculitis syndrome in children(Special under 5 years of age).KD has grown to be the main cause of childhood heart disease,which has beyond rheumatic heart disease.The severity of Kawasaki disease depends on the degree of coronary artery injury,some children followed coronary artery aneurysmeasily will progress to coronary artery obstructive expansion,causing myocardial ischemia and even myocardial infarction,resulting in sudden death of children and young adults.Kawasaki disease is also known as children’s "coronary heart disease." Intraveous immunoglobulin(IVIG)treatment is the most useful way to reduce the incidence of CAAS(Cornary artery abnormalities).If you can diagnose the disease and make appropriate treatment,the KD patients will get less risk of cardiovascular damage.But it is hard for the diagnosis of Kawasaki disease,especially early diagnosis,delayed diagnose will delay the treatment of IVIG in these children and result in coronary artery abnormalities and other cardiac complications in these children.In this study,we investigated the expression of IP-10(interferon-inducile protein-10),ApoA-1(apolipoprotein A1),ApoB(apolipoprotein B)and CD64 in diagnostic of Kawasaki Disease(KD).Methods and Results: In this study,we enrolled 31 children with fever and some clinical feathers of Kawasaki Disease.Among these children,16 children were diagnosed with Kawasaki Disease.The expression of IP-10,ApoA-1 and ApoB in plasma sample of these children was measured using ELISA.We also studied the expression of the CD64(One Fcγ-Receptor)on neutrophils and monocytes in these 16 KD children,15 Bacterial infections and 10 normal control children.The result showed that :(1)the IP-10 level of plasma sample is3062±298pg/ml at 1-3 days fever duration of KD children,and the IP-10 level in 4-8 days fever of KD children is 3466±211pg/ml,which is significantly higher than the IP-10 levels(973±150pg/ml)in control children with fever(P<0.05);(2)The level of Apo A-1 showed no significant difference between KD children and control children with fever;(3)The ApoB lever in plasma is 94.7±15.2 mg/dl at 1-3 days fever duration of KD children and 99.8±24.0 mg/dl,which is significantly higher than the ApoB level(71.5±15.1mg/dl)in control children with fever(P<0.05),(4)The number of CD64 molecules per cell was 13276±6531/neutrophil cell,46358±14589/monocyte cell,which was markly higher that the number in fevel group and control group(P<0.05).The number of CD64 molecules per neutrophil cell andmonocyte cell was 3029±10 and 22035±9960)in fevel group.In control group,the number was 1197±392 and 15072±6035,The data also indicated that after IVIG treatmet,the level of CD64 in neurophil and monocyte cells was reduce.Conclusion: The results showed that the expression of IP10,APOB and CD64 can be served as one potential biomarker for diagnosis.