The Active Ingredient Extraction Of Rhubarb In Qinghai

There is high medicinal in value Rhubarb, containing trace elements,anthracene deirvatives, stilbene compounds, tannins, organic acids, volatile oils, etc..Extraction and determination of the main ingredients of rhubarb are a lot, but thesystem reported little, so rhubarb extraction and determination of trace elements,tannins, organic acids, volatile oil, resveratrol did a full analysis and The extractionconditions were optimized, and laid the theoretical basis for the pharmacologicaleffect after the studying on rhubarb.First, the main contents1.Qualitativedetection by color reaction of tannin compounds and were determinedtotal content by complexometric titration and spectrophotonretric determinationtannins in rhubarb.2.Using acid-base titration method to the determine the total organic acid content inrhubarb,3.By CO₂-SFE to determine the total content of volatile oil in rhubarb and itsrerfactive index.4.By wet digestion-the atomic flame ionization spectroscopy of rhubarb and thesupercritical lfuid extraction of volatile oil K, Ca，Mg, Mn，Fe，Cu, Zn，Pb，Cr, Co，Cd，Ag12metallic elements content were measured at the same time.To do flirtherstudy of the metal content by ICP-MS method in rhubarb., in the determinationcontent of Li Be, B, Mg, Al，Ti，V, Cr, Mn, Co，Cu, Zn, Ga, As，CI, Se，Kr, Sr, Cd, Sb,Te，Ba，Au，Hg, Tl, Pb, Bi25elements.5.By color reaction, the IR spectra, UV analysis, liquid chromatography, qualitativeand quantitative test on the resveratrol in rhubarb.6.Investigated the rhubarb powder heat focusing on weight loss of400。C-1000。C.Two conclusions:1.The color reaction proves that it contains tannin compounds in rhubarb， usingcomplexometirc titration on the content of tannin compounds were determinedconclusions: the total content of tannins in rhubarb is12.45g/100g by heating relfux， it is12.63g/100g by extraction ultrasonic; it is12.41g/100g by spectrophotometry.Inthis study, single factor investigated the spectrophotometric, the best results is thesolvent70%acetone, ultrasonic extraction60min.2. Determination of the total organic acid content through orthogonal optimization inrhubarb, it shows that the extraction solvent95%ethanol, the ultrasound30min，Mateiral: the extraction solvent is1:20; total organic acid content is4.83g;3.By orthogonal test to optimize the content of the rhubarb volatile oil extractionconditions: the highest volatile oil content is extraction pressure is25Mpa, extractiontemperature is450C，extraction time is90min, the flow of CO₂is17.5L/h?The volatile oil content0.154g/100g, the rerfactive index of the volatile oil was1.4831in rhubarb;4.By wet digestion-atomic flame ionization spectroscopy found, the12kinds ofmetal elements, K, Ca，Mg, Fe，Zn in the rhubarb with higher levels of highercontent of more than lOOug/g，not found Pb, Ag two elements in rhubarb; byICP-MS analysis showed that in the25kinds of elements in rhubarb, and Mg, Al, Srcontent exceeds100mg/L; heavy metals and toxic elements of Be, V，Cr, As，Se,Cd，Hg, Pb content is below1Omg/L.5.It contains resveratrol in rhubarb，and the structure of resveratrol is main cisstructure in rhubarb; the resveratrol’s content is98.938%（cis-resveratrol，69.474%ï¼›trans-resveratrol,29.464%） in extract; its content is2.352%o in rhubarb;6.In this temperature range of rhubarb powder at400°C-1000°C TGA spectrum canbe divided into four stages.