Effects Of Guizhi Fuling Capsule On The Atherosclerosis And The Microvascular

Backgroud Atherosclerosis disease is seriously harmful to human health because of its high mortality and high morbidity; coronary microcirculation lesion in the atherosclerosis. It has been confirmed that Guizhi Fuling Capsule has the effects of anti-atherosclerosis, but it has not been reported the mechanisms and effects of coronary microvascular.Objective To observe the anti-atherosclerosis and the microvascular effects of Guizhi Fuling Capsule and analyze its mechanisms in atherosclerosis(AS) rats, and to provide the evidence for the clinical treatment.Methods Healthy male SD rats were randomly divided into two groups:normal group, AS model group. The model of experimental AS rats was established by high cholesterol diet and intraperitoneal injecting Vit D3. The normal control group was fed by ordinary diet.AS model group was randomly divided into3groups AS group, control group, trentment group. Treatment group were given Guizhi Fuling Capsule8ml/kg·d(0.24g/kg·d)by intragastric administration.Control group were given simvastatin(0.86mg/kg·d)and captopril(4.3mg/kg·d) by intragastric administration. The AS group were0.9%NaCl instead of Guizhi Fuling Capsule. The rats were killed at the14th weekend and breast aorta、myocardium were observe by paraffin sections. Hematoxylin and eosin staining was made in order to observe the formation of pathological change in breast aorta under the microscope. Serum level of Nitric oxide (NO), Endothelin-1(ET-1), high-sensitivity C-reactive protein (hs-CRP) were detected respectively. Observing the expression of Matrix metalloproteinase-2(MMP-2),Tissue inhibitor of metalloproteinas-2(TIMP-2) and Nuclear transcription factor KappaB(NF-κB) in myocardium by Immunohistochemistry technology. Results(1) The aorta of normal group rats was smooth under light microscope. Typical atherosclerotic plaques in AS group could be found, but they weren’t found in treatment group and control group.(2) The level of NO in serum:The level of NO in AS group rats were lower than that in normal group(P<0.01), and the level of NO in treatment group were higher than that in AS group and control group(P<0.05). The level of ET-1in serum:The level of ET-1in AS group rats were higher than that in normal group(P<0.01), and the level of ET-1in treatment group were lower than that in AS group (P<0.01). There was no significant difference of NO in serum between treatment group and control group(P>0.05).(3) The level of hs-CRP in serum:The level of hs-CRP in AS group rats were higher than that in normal group(P<0.01), the level of hs-CRP in treatment group were lower than that in AS group (P<0.05). There was no significant difference of hs-CRP in serum between treatment group and control group (P>0.05)(4) The density of microarteries in myocardium:The density of microarteries in AS group rats were higher than that in normal group(P<0.01), the density of microarteries in treatment group were lower than that in AS group (P<0.05). There was no significant difference between treatment group and control group (P>0.05). The density of capillaries in myocardium:The density of capillaries in AS group rats were lower than that in normal group(P<0.01), the density of capillaries in treatment group were higher than that in AS group (P<0.05). There was no significant difference between treatment group and control group (P>0.05)(5) The level of MMP-2in myocardium:The level of MMP-2in AS group rats were higher than that in normal group(P<0.01), The level of MMP-2in treatment group were lower than that in AS group (P<0.05). There was no significant difference between treatment group and control group (P>0.05). The level of TIMP-2in myocardium:The level of TIMP-2in AS group rats were higher than that in normal group(P<0.05), The level of TMP-2in treatment group were higher than that in AS group (P<0.01). There was no significant difference between treatment group and control group (P>0.05). The level of NF-κB in myocardium:The level of NF-κB in AS group rats were higher than that in normal group(P<0.01), The level of NF-κB in treatment group were lower than that in AS group (P<0.05). There was no significant difference between treatment group and control group(P>0.05).Conclusions Guizhi Fuling Capsule has the effects of Anti-atherosclerosis and can improve the microcirculation with coronary artery to rats with AS. The mechanism of Anti-atherosclerosis maybe relate to its functions of holding the balance of NO/ET-1, anti-inflammatory effect and protection of endothelial function. They can improve the microcirculation with coronary artery, because that can change the density of microarteries and capillaries, adjust the level of MMP-2, TIMP-2and NF-KB.