Expression And Significance Of Kiss-1、NF-κBp65、MMP-9、CD44v6and E-cadherin In Placental Site Trophoblastic Tumor

Objective:PSTT is a less common type in gestational trophoblastic disease, deriving from intermediate trophoblastic cell. Most of these tumors don’t metastasis, and have good prognosis; but there is about15%～30%cases metastasizing,once metastasized,there usually is extensive dissemination and bad prognosis, and if no treatment in time,the mortality is about10%～20%[1]. PSTT which have not metastasis need operation, but which metastasis need operation and chemotherapy. So far, the mechanism of PSTT has been not clear.There has been not a method that can predicte the infiltration and metastasis in PSTT. Our target is to explore the expression and correlation of kiss-1, NF-kbp65, MMP-9, CD44v6, E-cadherin in PSTT by immunohistochemistry,Methods:We investigated formalin-fixed paraffin embedded PSTT specimens (n=10), the average age is30years, the prepregnancy villus (n=20)(5-12weeks); the hydatidiform mole(n=15), the chorioepithelioma (n=15). We investigated the expression of kiss-1, NF-κbp65, MMP-9, CD44v6, E-cadherin in PSTT, prepregnancy villus, chorioepithelioma. The data was analyzed by Kruskal-Wallis H, Mann-Whitney U and Spearman.Results:1. No significant difference of Kiss-1expression were found between the normal chorionic villi of5-12weeks and that of hydatidiform mole (P>0.05). However, there was a significant reduction of Kiss-1expression in the tissues of chorioepithelioma and PSTT compared with that of5-12weeks chorionic villi and hydatidiform mole(P<0.05). But there was no significant difference between PSTT and chorioepithelioma (P>0.05).2. There was a significant increasion of NF-κBp65expression in the tissues of chorioepithelioma and PSTT compared with that of5-12weeks chorionic villi and hydatidiform mole(P<0.01).And there was significant different NF-KBp65expression between PSTT and chorioepithelioma(P<0.01).3. There was a significant increasion of MMP-9expression in the tissues of chorioepithelioma and PSTT compared with that of5-12weeks chorionic villi and hydatidiform mole(P<0.01).But no significant difference of MMP-9expression were found between PSTT and chorioepithelioma(P>0.05).4. There was a significant increasion of CD44v6expression in the tissues of chorioepithelioma and PSTT compared with that of5-12weeks chorionic villi and hydatidiform mole(P<0.01). But no significant difference of CD44v6were found between PSTT and chorioepithelioma(P>0.05).5. There was a significant reduction of E-cadherin expression in the tissues of chorioepithelioma and PSTT compared with that of5-12weeks chorionic villi and hydatidiform mole (P<0.05). However, no significant difference of E-cadherin expression were found between the normal chorionic villi of5-12weeks and that of hydatidiform mole (P>0.05). But there was no significant difference between PSTT and chorioepithelioma (P>0.05).6. The expressions of Kiss-1and NF-κBp65,MMP-9, CD44v6in all groups were negatively correlated; however Kiss-1and E-cadherin were positively correlated.Conclusions:1. The results of the studying suggests that there is significant difference during PSTT comparing with chorionic villi at the level of expression. And the expression level between chorionic villi, hydatidiform mole and chorioepithelioma. It suggests that the indexes take part in the pathogenesis of PSTT. It is consistent with the biological behavior of PSTT.2. Kiss-1and NF-Kbp65, MMP-9were negatively correlated, Kiss-1may affect the process of the metastasis and infiltration of PSTT, it may regulate the transcription and/or expression of NF-Kbp65and MMP-9.3. The expressions of Kiss-1and E-cadherin in PSTT were positively correlated, kiss-1may regulate the the transcription and/or expression of E-cadherin, so it may take part in the adhesion of tumor cells.4. The expressions of NF-κBp65. MMP-9and CD44v6in all groups were positively correlated, it may mean that as downstream genes which are regulated by Kiss-1may interact with each other at the level of gene or protein. And the interaction personificate the mechanism of the infiltration and metastasis of tumors.5. The further study of kiss-1can help the understanding of the infiltration and metastasis of PSTT. It interfers the transcription and expression of kiss-1in tumor cells at the level of molecular, it may be better than regulating downstream genes in the effect in inhibiting the infiltration and metastasis of tumors.