Study On The Influence On The Free Radical Metabolism Of HLP Mice By Swimming In Low Temperature

Objective:We Observed the effects of different forms and different periods of swimming exercise on hyperlipidemia (HLP)rat’s serum levels of MDA content and SOD,GSH-PX, T-AOC activity. Study whether the low water temperature swimming on hyperlipidemia (HLP) rats’s balance regulation of the free radicals of generation and scavenging to reduce the body’s lipid peroxidation;from the perspective of antioxidant to comparison which patterns of a low water temperature swimming or swimming at room temperature to prevent and treatment of the cerebrovascular disease’effect is better; Discussion that how long the period of exercise to adapt to the movement of winter swimming exercise. To provide a scientific basis for Hyperlipidemia (HLP) symptoms group to establish a scientific and reasonable winter swimming exercise prescription.Methods:Wistar male rats were 64,7-8 weeks old, randomly divided into the normal control group (16) and the model group of hyperlipidemia (48),Divided normal control group into normal control group of 4 weeks group (NC1) and the normal control group of 8 weeks group (NC2) ,divided the model group of hyperlipidemia into hyperlipidemia control 4 weeks group (HC1),hyperlipidemia control 8 weeks group (HC2), swimming at room temperature 4 weeks group (NS1), swimming at room temperature 8 weeks group (NS2), low-temperature swimming 4 weeks group (LS1), low-temperature swimming 8 weeks group (LS2), After the successful model of hyperlipidemia;measured content of serum MDA by ELISA; measured activity of serum SOD, GSH-PX, T-AOC by ELISA, measured four lipids (HDL, LDL, TG and TC). By automatic biochemical analyzerResults:1、Hyperlipidemia control 4 weeks,8 weeks group of TG, TC, LDL were significantly higher than the normal control group 4 weeks,8 weeks (P<0.01), hyperlipidemia control 4 weeks group, 8 weeks group of HDL were significantly lower than the normal control group 4 weeks, 8 weeks group (P<0.05). 4 weeks of low temperature swimming group,s content of TG, TC, LDL below the swimming at room temperature4 weeks group (P<0.05) lower the hyperlipidemia control 4 weeks group (P <0.01),while the low-temperature swimming 4 weeks group,s content of HDL higher than 4 weeks of swimming at room temperature group (P<0.05) higher than that of hyperlipidemia control 4 weeks group (P<0.01), low-temperature swimming 8 weeks group,s content of TG, TC, LDL less than eight weeks of swimming at room temperature group (P<0.05) less than 8 weeks of hyperlipidemia control group (P<0.01) while low temperature swimming 8 weeks group,s content of HDL higher than 8 weeks of at room temperature swimming group (P <0.05) higher than the 8 weeks of hyperlipidemia control group (P<0.01).8 weeks of low temperature swimming group ,s content of TG, TC, LDL than the group of low temperature swimming of 4 weeks had decreased, but no significant difference (P>0.05), 8 weeks of low-temperature swimming group’s content of HDL than the group of low temperature swimming of 4 weeks had tended to increase but no significant difference (P>0.05). Swimming at room temperature of 8 weeks group TG, TC, LDL than at room temperature of 4 weeks group content of MDA p TG, TC, LDL had decreased, but no significant difference (P>0.05), swimming at room temperature of 8 weeks group HDL than swimming at room temperature of 8 weeks group has tended to increase, but no significant difference (P> 0.05).2、Hyperlipidemia control 4 weeks, 8 weeks group’s content of MDA was significantly higher than the normal control 4 weeks, 8 weeks group (P<0.01), hyperlipidemia control 4 weeks group’s content of MDA higher than Swimming at room temperature of 4 weeks group ( P <0.01), hyperlipidemia control 8 weeks group’s content of MDA higher than Swimming at room temperature of 8 weeks group ( P <0.01), Comparison of swimming at room temperature of 4weeks and 8 weeks, swimming at room temperature of 8weeks group had tend to decreased but no significant difference(P>0.05).Comparison of hyperlipidemia control 4 weeks group’s content of MDA and the low temperature swimming of 4 weeks, low temperature swimming of 4 weeks had tend to decreased ,but no significant difference(P>0.05); Comparison of low temperature swimming of 4 week group’s content of MDA and swimming at room temperature of 4weeks ,slightly higher, but no significant differenc(eP>0.05); hyperlipidemia control 8 weeks group’s content of MDA ,higher than low temperature swimming of 4 weeks(P<0.05), low temperature swimming of 8 weeks group’s content of MDA lower than swimming at room temperature of 8week(P<0.05). low temperature swimming of 8 weeks lower than low temperature swimming of 4 weeks group’s content of MDA(P<0.05).3、Hyperlipidemia control 4 weeks, 8 weeks group’s activity of SOD was lower than the normal control 4 weeks, 8 weeks group (P<0.01), swimming at room temperature of 4weeks group’s activity of SOD higher than hyperlipidemia control 4 weeks(P<0.01), swimming at room temperature of 8weeks group’s activity of SOD higher than hyperlipidemia control 8 weeks(P<0.01), swimming at room temperature of 8weeks group’s activity of SOD slightly higher than swimming at room temperature of 4weeks, but no significant difference(P>0.05). low temperature swimming of 4 week group’s activity of SOD lower than swimming at room temperature of 4weeks(P<0.05),and hyperlipidemia control 4 weeks(P<0.01). low temperature swimming of 8week group’s activity of SOD higher than hyperlipidemia control 8 weeks(P<0.01),low temperature swimming of 8week group’s activity of SOD slightly higher than swimming at room temperature of 8weeks, but no significant difference(P>0.05)low temperature swimming of 8week group’s activity of SOD slightly lower low temperature swimming of 4 weeks group, but no significant difference(P>0.05).4、Hyperlipidemia control 4 weeks, 8 weeks group’s activity of GSH-PX was lower than the normal control 4 weeks, 8 weeks group (P<0.01), low temperature swimming of 4week group’s activity of GSH-PX higher than swimming at room temperature of 4weeks(P<0.05)higher than hyperlipidemia control 4 weeks(P<0.01), low temperature swimming of 8 week group’s activity of GSH-PX higher than swimming at room temperature of 8weeks(P<0.05)higher than hyperlipidemia control 8 weeks group(P<0.01), low temperature swimming of 8 week group’s activity of GSH-PX slightly higher than low temperature swimming of 4 weeks group, but no significant difference(P>0.05)swimming at room temperature of 8weeks group’s activity of GSH-PX slightly higher than swimming at room temperature of 4 weeks group, but no significant difference(P>0.05).5、Hyperlipidemia control 4 weeks, 8 weeks group’s activity of T-AOC was lower than the normal control 4 weeks, 8 weeks group (P <0.01), low temperature swimming of 4week group’s activity T-AOC of higher than swimming at room temperature of 4weeks(P<0.05)higher than hyperlipidemia control 4 weeks(P<0.01), low temperature swimming of 8 week group’s activity of T-AOC higher than swimming at room temperature of 8weeks(P<0.05)higher than hyperlipidemia control 8 weeks group(P<0.01), low temperature swimming of 8 week group’s activity of T-AOC slightly higher than low temperature swimming of 4 weeks group, but no significant difference(P>0.05)swimming at room temperature of 8weeks group’s activity of T-AOC slightly higher than swimming at room temperature of 4 weeks group, but no significant difference(P>0.05)Conclusions:1、The contion of hyperlipidemiat ,the generating of rat’serum content of MDA has increase, rat’serum activity of SOD, GSH-PX, T-AOC has decreased, indicating that at the condition of hyperlipidemiat, free radicals‘s lipid peroxidation increased and promote the formation of atherosclerosis. 2、Each group of hyperlipidemia , exercise group reduced the level of blood lipid, the formation of MDA decreased, the activity of SOD, GSH-PX, T-AOC increased, indicating that exercise can reduce blood lipid, increased the capacity of the body’s Clear free radical and Reduce the damage to body.3、After the establishment of Adaptation to Exercise, the antioxidant capacity of hyperlipidemia in rats, Long period Exercise better than the short period Exercise; low temperature swimming group better than t swimming at room temperature group. To a certain degree,winter swimming can prevent the happening and development of hyperlipdemia and CCVD.