Study On The Separation Preparation And Antioxidant Of Camellia Pericarp Polyphenol

In China, Camellia has abundant resources and abroad distribution, but the report about Camellia pericarp polyphenol was rare. Biophenol has various bioactive, it has funtions in improving plant oil quality, steady, health protection. First the content of Camellia pericarp polyphenol was determined, then isolated and made component analysis, after that collected fractions and carried out their anti-oxidation, the main results were as follows:1. Folin-ciocalteu method was used for determination of polyphenol content in Camellia pericarp polyphenol, The result of method evaluation showed that, stability was well, absorbance was close to steady after60min, RSD of reproducibility experiment was0.539%, RSD of precision experiment was0.386%, the recovery was between97.05%-100.1%. So this method can be used for determination of polyphenol content in Camellia pericarp polyphenol, the result was accurate and reliable. The method was used to determine four kinds of Camellia pericarp polyphenol which were grew up in the same place and picked at the same time, the results showed that the polyphenol content was different, it declared that there was diversity between the varieties.2. SPE technic and HPLC method were used to isolate and determinate crude extraction of Camellia pericarp polyphenol, the optimization condition was as follows:Supel clean TM LC-18SPE Tubes(3mL,500mg) were choosed to isolate polyphenol, the solid-phase extraction column was activated successively by6mL methanol、6mL acetonitrile、10mL water, the sample was absorbed completely, then collected the elution, analysed by HPLC. According to the HPLC analysis of four kinds of Camellia pericarp polyphenol was different which purified by SPE technic, the results showed that: compared with the sample, Camellia japonica and Camellia chekiangoleosa Hu. may have tannic acid, gallic acid, catechin and rutin, Camellia polyodonta How. Eχ Hu. may have tannic acid and catechin, Camellia semiserrata Chi. may have catechin. 3. The crude elution of Camellia pericarp polyphenol was isolated and prepared through semi-preparation HPLC after purified by SPE technic, the optimization condition of semi-preparation HPLC was as follows:gradient elution was carried on by mobile phase A (water:acetic acid=100:1) and B (acetonitrile) at2.5mL/min flow rate, the injection volume was0.4mL. Eight fractions were collected, the content of fraction E which collection time was between18.30-18.80min has the highest polyphenol content, was13.54mg/g.4. Anti-oxidation of eight fractions collected with semi-preparation HPLC were determined through DPPH and ABTS methods, the results showed that, the results of two methods were consistent, there were five fractions had high oxidation resistance, the structure of them needed further studies. The relevance of polyphenol content and anti-oxidation was studied, the result showed that they presented straight relevance.