Association Of FGFR2 Polymorphisms With The Risk Of Breast Cancer In Chinese Women Of Ningxia Han Population

Objective To estimate the relative risk and contribution of FGFR2 polymorphisms to breast cancer risk in Chinese women of Ningxia Han population, and test the association of the haplotypes that composed by 4 SNPs with breast cancer risk. To investigate the potential association of FGFR2 polymorphisms and clinicopathologic characteristics with breast cancer risk in Chinese women of Ningxia Han population.Subjects and Methods The case-control study included 203 female sporadic breast cancer cases and 200 cancer-free controls. Both cases and controls were consecutively recruited from the First Affiliated Hospital of Ningxia Medical University between November 2008 and May 2010. All cases were newly diagnosed and histopathologically confirmed. The average age of case group was 50.0±9.2 years and the control group was 47.2±9.7 years. Cancer-free control women frequency-matched to the cases of age (±5 years). All participants were genetically unrelated ethnic Han Chinese women, and permanent residents of Ningxia Region. The PCR-PFLP assays were conducted to detect the genotype of rs2981579,rs1219648,rs2420946 and rs2981582. SHEsis online software was used to construct the haplotypes for identified polymorphisms. Descriptive statistical analysis was performed by using SPSS 13.0 software, usingχ~2 test for categorical variables and t-test for continuous variables.Result 1. There was significant difference of rs2981582 genotype distribution between cases and controls(χ~2=7.696,P=0.021), but no evidence of significant associations between risk of breast cancer and other 3 SNPs in FGFR2. There were significant differences of rs1219648,rs2420946 and rs2981582 genotype distribution in postmenopausal women between case and groups (P < 0.05). 2. There was no significant association of clinicopathologic characteristics (ER, PR status) with breast cancer risk (P > 0.05). 3. Haplotype analysis identified 6 common haplotypes with frequencies greater than 0.03. Common risk haplotypes CGCT (OR, 0.61; 95% CI, 0.46– 0.81; P = 0.001) significantly associated with reduce breast cancer risk, and TGTC (OR, 6.56; 95% CI, 2.06– 20.85; P = 0.000) and CATC (OR, 12.65; 95%CI, 1.74– 91.80; P = 0.001), was significantly associated with increase the breast cancer risk.Conclusions 1. FGFR2 rs2981582 might associate with breast cancer risk in Ningxia Han population; rs1219648,rs2420946 and rs2981582 polymorphisms might associate with breast cancer risk in postmenopausal women of Ningxia Han population. 2. There was no significant association of clinicopathologic characteristics (ER, PR status) with breast cancer risk in Ningxia Han population. 3. Among the haplotypes composed by 4 SNPs in FGFR2, the CGTC haplotype might be the protective factor of breast cancer; TGTC and CATC haplotypes may be the risk factor of breast cancer.