Acupuncture Combined With The Ean Rabbit Peripheral Nerve Pathological And Electrophysiological Features Of The Study

Purpose: Through needle medicine combined on the Guillain-Barre syndrome classic animal model - experimental allergic neuritis (EAN) in rabbit peripheral nerves and nerve pathomorphology electrophysiological function study.Method: 12ml extract of bovine myelin basic protein II (P2) with the contour of complete Freund's adjuvant (CFA) to fully prepare a mixture of immune rabbit animal model of EAN. 16 normal, healthy rabbits as a normal group, normal group to 12ml with normal saline in place of P2 with the cow equivalent CFA immunization as a control, another successful model from 64 EAN rabbits were randomly divided into normal group, model group, Tongjing Yisui side group, needle medicine combined group immunoglobulin group, 16 in each group. Tongjing Yisui side group Tongjing Yisui side nasal feeding treatment; needle medicine combined pass through electric acupuncture to Shu-points of the five-organs and Tongjing Yisui side;immune globulin group immunoglobulin 40mg / (kg ? d) treatment by intraperitoneal injection. In the 7th days and the 14th days we separately examined each group of experimental animal sciatic nerve movement conduction velocity and the F wave appearance frequency, and selected the material from the experimental animal, and used the electricity to observe the change of the peripheral nerve pathology morphology ultrastructure, application of electron microscopy the ultrastructure of sciatic nerve pathological changes, and optical microscopy.Result: 1 The success of this experiment replicated EAN rabbit model, in terms of pathology, neuro-electrophysiology is consistent with the relevant reports to prove that this experimental study in the animal model is successful.2 Needle medicine combined group can improve very effectively has the marrow nerve fiber to have the cavitation, the thick in and out and the line plastochondria is tumescent, causes to escape the myelinic membrane change not to be obvious, sheath level readability enhancement. Under the light microscope sees the phlogocyte infiltration to reduce.3 In neural electrophysiological, the needle medicine combined group can motor nerve conduction velocity increased significantly faster, F-wave abnormalities were significantly reduced, significant improvement in nerve conduction block phenomenon. Conclusion: 1 Needle medicine combined can significantly improve the myelinated nerve demyelination and inflammatory cell infiltration.2 It can increase the motor nerve conduction velocity to improve the conduction block phenomenon after needle medicine combined.3 Needle medicine combined is based on combining the theory as a guide through the pulp, the best starting point for governance atrophy.