Ea Bladder Through The Five Internal Organs Yu Impact Of Guillain - Barre Syndrome Rabbit Sciatic Nerve

Objective:To observe the influence of electroacupuncture at Shu-points of the five Zang-organ for the GBS rabbit's sciatic nerve pathology morphology ultrastructure and sciatic nerve organizes Helper T cell subsets TNF-α, IL-12mRNA expression level; to discuss the function of Helper T cell subsets in the mechanism of GBS; thus, to promulgate the mechanism of the treatment of GBS by electroacupuncture at Shu-points of the five Zang-organ.Method: This experiment totally used 80 domestic rabbits which divided into four groups: blank group,model groups, the electroacupuncture group, the immunity globulin group, each group had 20 cases. The electroacupuncture group gave electroacupuncture at Shu-points of the five Zang-organ and the immunity globulin group gave the rabbit immunity globulin 80mg/(kg·d) intravenous injection.In the 7th days and the 14th days we selected the material from the experimental animal,and used the electricity to observe the change of the peripheral nerve pathology morphologyultrastructure; using RT-PCR to examine the expression level of Helper T cell subsets TNF-α,IL-12mRNA.Result1. We observed the model groups domestic rabbit sciatic nerve mainly pathology performance is myelin sheath swelling and myelinoclasis, with the chondriosome tumescent, the schwann cell nucleus pyknosis, the cytoplasmic matrix cavitation, the cell organ swelling. After the treatment, the electroacupuncture group and the immunity globulin group'smyelinoclasis status had a distinct improvement, and the readability of myelin sheath lamina had a enhancement, with the thick nerve fiber structure basically normal, with the chondriosome individual tumescent, the schwann cell organ swelling decrease, cellular form basically restoration, newly tiny marrow nerve fiber production.2. In the model groups, the mRNA expression of TNF-α, IL-12 obviously advanced, while after the treatment it dropped obviously. There is no significant differences between the electroacupuncture group and the immunity globulin group (P＞0.05) in the 14th day. Conclusion1. The myelin sheath swelling and myelinoclasis of GBS domestic rabbit sciatic nerve got improved after electroacupuncture at Shu-points of the urinary bladder five Zang-organs, while the chondriosome and the schwann cell organ swelling reduced, the cellular form restored basically.2. Helper T cell subsets existed unbalance in GBS. Electroacupuncture at Shu-points of the urinary bladder five Zang-organs could antagonist the unbalance of Th cell subsets and caused the TNF-α,IL-12 mRNA expression to reduce obviously...