| Objective To investigate the correlation of plasma AHSG level and lower extremity atherosclerosis (LEA) in type 2 diabetes complicated with renal insufficiency.Methods Two hundred and twenty six type 2 diabetes were enrolled. Severity of lower extremity atherosclerosis was examined by Color Doppler Ultrasound of Alokaα10 (Japan). Plasma AHSG level was measured by enzyme linked immuno sorbent assay(ELISA). Glomerular filtration rate was calculated with Cockcroft-Gault equation according to the guideline of K/DOQI of National Kidney Foundation (NKF). The subjects were divided into normal (n=81), mild renal insufficiency (n=46) and moderate to severe renal insufficiency (n=99) groups.Results①There were significant differences in LEA incidence (59.9%vs18.5%vs22.7 %), LEA scores (1.27±0.32vs0.52±0.31vs0.83±0.36) and plasma AHSG levels (0.43±0.26g/Lvs0.59±0.39 g/Lvs0.72±0.54 g/L) among moderate to severe renal insufficiency, normal and mild renal insufficiency groups (P<0.01).②There was no correlation between plasma AHSG levels and LEA (r=0.028, P=0.803; r=-0.158, P=0.294) in normal and mild renal insufficiency groups, whereas there was a positive correlation between plasma AHSG levels and LEA (r=0.592, P< 0.001) in moderate-severe renal insufficiency group.③Multiple stepwise regression analysis showed both HBA1c,TC,TG,GFR and AHSG were primary factors of LEA in moderate-severe renal insufficiency group. Conclusions①Compared with normal and mild renal insufficiency groups, the incidence and severity of LEA were higher in moderate-severe renal insufficiency group.②Plasma AHSG level can be used as a measurable marker of LEA severity in type 2 diabetes complicated with moderate-severe renal insufficiency. Objective To determine the correlation of four SNP genotype sites of AHSG (rs4917,rs4918,rs1071592,rs2248690) in type 2 diabetes and artery of lower extremity atherosclerosis (LEA) in type 2 diabetes in Kunming Han Chinese.Methods Applied the case-control method, we detected the four SNP genotype sites of AHSG in eighty eight normal controls, eighty four type 2 diabetes cases and one hundred and sixty one LEA in type 2 diabetes cases in Kunming Han Chinese by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) and allelic specific primer-polymerase chain reaction(ASP-PCR), analysed the frequency of four SNP genotype and allele and relative clinical data were compared between groups. Results①There were no significant differences on frequency of genotype and allele distributions in the polymorphism analysis of rs4917(C/T),rs4918 (C/G),rs2248690(A/T) between the cases and controls (P>0.05).②There were significant difference on freguency of genotype and allele distributions in rs1071592(C/A)(x2=13.505,P<0.001 and x2=5.193, P=0.023) among normal controls, type 2 diabetes cases and LEA in type 2 diabetes cases the groups.③The C/A and A/A genotypes of rs1071592 would be the risk factors in type 2 diabetes [OR=8.501,95%CI:1.210-60.153].④The risk would be relatively increased in the people who carried the C/A and A/A genotypes of rs1071592 with LEA in type 2 diabetes [OR=2.235,95%CI:1.107-4.516]. Conclusions①The two methods of PCR-RFLP and ASP-PCR were stability, reproducibility, specificity and accuracy for SNP somatotype.②There were existed polymorphism of rs4917(C/T), rs4918(C/G), rs1071592 (C/A), rs2248690(A/T) of AHSG in Kunming Han Chinese.③The C/A and A/A genotypes of rs1071592 would be the risk factors in type 2 diabetes and LEA in type 2 diabetes. Objective To assess the association between type 2 diabetes with lower extremity Artherosclerosis (LEA) and the change of bone mineral density. Methods Two hundred and nineteen type 2 diabetes were enrolled.Severity of LEA was examined by Doppler Ultrasound of Aloka-α10 (Japan).The bone mineral density(BMD)in lumbar vertebrate (L1-L4) and femoral bone was measured by Dual-energy X-ray absorption-metry(DEXA) made in America.According to severity of LEA score, the subjects were divided into the control(n=65),mild(n=62), moderate (n=56)and severe(n=36) groups. Results①There was difference on the HBA1c,FBG,2HPBG,TC,TG,HDL,LDL among groups(P<0.05或P<0.01).②The age,duration of diabetes,complicating rate of hypertension,SBP,HBA1c,FBG,2hPBG,TC,TG,HDL,LDL were positive correlation with the lower extremity atherosclerotic severity score (P<0.01), but the BMI,HDL were negative correlation with it(P<0.05 or P <0.01).④The BMD in lumbar vertebrate(L1-L4) and femoral bone were negative correlation with the lower extremity atherosclerotic severity score.⑤By multivariate Logistic regression analysis showed the change of BMD were primary risk factors of LEA in type 2 diabetes (OR=22.762,95%CI:3.752-68.461).Conclusions①The age,duration of diabetes,complicating rate of hypertension,SBP,HBA1c,FBG,2hPBG,TC,TG,LDL were the risk factors of lower extremity artherosclerosis of type 2 diabetes mellitus,while HDL was protect factor,so the patient with type 2 diabetes should control actively the blood glucose,blood lipids and blood pressure to standard.②The LEA of type 2 diabetes mellitus was immediate-ly correlation with the change of BMD. The degree of LEA was more severe and the change of BMD more obvious. The descent about BMD was risk factors of LEA production and development. |
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