5 - Ht 1a, 2a Receptor Gene Expression In The Hypothalamus In The Rhesus Monkey Model Of Premenstrual Syndrome Research

PMS (premenstrual syndrome) is a series of physical and emotional uncomfortable symptoms that some women have during premenstrual stages, mainly including fidgety, irritability, insomnia, tension, depression, headache, breast swelling and so on. Normal life of women could be seriously affected by PMS. Although statistic of the incidence of PMS varies when different diagnostic standards and methodology were used, it is considered generally 75-95% women have slight symptoms, 20-40% around reach the diagnosis standards, 2-15% can be diagnosed as to be serious. PMS bring social disserves as serious as depression. Although the incidence of the disease is very high, the cause of specific mechanisms is not yet clear.In our previous study, we constructed a subtractive cDNA library from the cDNA of marginal brain system of PMS model macaque after subtractively hybridized with normal macaque cDNA. Candidate genes presumably related to PMS were obtained from the library, encoding some neurotransmitter receptors, such as the GABA, 5-HT_1A and 5-HT_2A receptors. 5-HT_1A and 5-HT_2A receptor genes from macaque have very high level of homology with those from human. RT-PCR was employed to get key sex hormone receptor genes, for example, the estrogen receptors (ER-α and ER-β) and progesterone receptor (PGR) genes that may have close relationship with PMS. Total RNA was isolated and purified from the brain marginal...