The Musk Yellow Xiaoliu Side Inhibition Of Experimental Study, H22 Mice Liver Cancer Angiogenesis And Anti-metastasis Effect

Objective To study the effect of SheHuangXiaoLiuFang ( abbreviated as SHXLF) in treatment of invasion and metastasis in mice with transplanted liver cancer(H22), especially to observe the effect of SHXLF on VEGF (Vascular Endothelial Growth Factor) content and MVD (microvascular density) on mice transplanted hepatocarcinoma, so as to make sure the mechanism of anti-invasion and metastasis of SHXLF on the mice hepatocellular carcinoma, to provide foundation for the clinic appliance of SHXLF.Methods 75 male mice ( SPF ) were transplanted liver cancer(H22) subcutaneously into left footpads to be established as experimental lymphatic metastasis models, and were divided randomly into 5 groups(each group had 15 mice): the pathologic model group(group A), SHXLF low, middle, high dose treatment groups (group B, C, D), the western medicine group (group E). From the next day, the groups were treated by SHXLF low, middle, high dose and 5-Fu respectively, the pathological group was given the same dose NS (Normal Saline) , Once everyday. After 20 days, the mice were killed and weighed. The transplanted lateral popliteal fossa, para-iliac artery and renal lymph nodes, thymuses, spleens, and their left footpads were excavated, weighed, and some parts of them were performed on parafomaldehyde-fixed, paraffin-embedded sections. Count the index of lymph nodes, thymuses, spleens. The invasion and metastasis occurring in footpads and lymph nodes were observed and computed, the expression of VEGF, MVD were also measured with immunohistochmeistry staining.Results Compard with pathological model control group, the SHXLF treatmentgroups can decrease the level of invasion and metastasis in footpads and popliteal fossalymph nodes of hepatocellular carcinoma, the index of lymph, and the high dose group(group D) were significantly decreased(P<0.05). The SHXLF middle, high dose groups(group C,D) can significantly increase index of thymus(P<0.05), the western medicinecontrol group( group E) decreased the index of spleen(P<0.05), while the SHXLF groupsdidn't have the side-effect(P>0.05). On the other hand, the group B, C, D cansignificantly decrease the VEGF content and MVD.Conclusion 1) The SHXLF can inhibit the invasion of the hepatocellular carcinoma in mice's footpads and lymph nodes, and it can decrease the index of lymph node, prevent lymphatic metastasis.2) Its mechanism is to improve immune functions and injury the carcinoma cell, to decrease VEGF content and MVD in liver cancer, so as to inhibit the tumor angiogenesis. And the effect can be unproved with the increase of SHXLF dose in proper range. 3) It seems that the dose should be higher to inhibit the proliferation and the tumor angiogenesis; while the dose should be lower to inhibit the metastasis, recurrence, and aggravation of hepatocellular cancinoma.