| Acute pain after traumatic were a serious acute stress reaction whether in war or peace-time. In recent years, with upgrading of the weapons and the warfare, acute traumas in wartime become more serious and complicated. In peacetime, high-energy trauma caused by traffic accidents or natural disasters is also very common. Bleeding and pain are two major characteristics common to such injuries. It is often difficult to control life-threatening bleeding; simultaneously severe pain of trauma brings great physical and mental effects increasing the difficulty of treatment. Early analgesia of acute post-traumatic pain is an important part of first aid, which is benefit to treatment and prognosis for the wounded. Previously our department has done some research on hemostatic agent, in order to develop a new type of hemostat for combat first aid. On this basis, this project is hope to make effective interventions for acute pain while effectively control of bleeding after trauma. Objective: 1, To develop an appropriate animal models for topical analgesic pain medications; 2, To find a suitable topical analgesics as one of Hemostat components ,which would not reduce the effect of the hemostat; 3, To specify the concentration of the topical analgesics.1. A rodent model of hind limb cut Wound Injury for acute painTwo models were made in 23 male adult SD rats. In Beitz's hind limb pain model, an incisional stab wound was placed on the calf, causing both superficial and deep tissue injury. In the improved model, the incision on the calf which deepened to the fascia and muscle was extended from 5mm to 10mm, the rest steps is as same as the Beitz model. The animals were randomly divided into 3 groups: control group (C n=3), Beitz model group (AM n=10), improved Beitz model group (IM n=10). After modeling, experimental animals were measured secondary mechanical pain threshold and pain threshold, and compared. The results showed that the improved Beitz model group which can simulate the occurrence and development of acute pain, was suitable for evaluating the analgesic effect of local analgesia.2. The analgesic effect of compound medicines about Zeolite type 5A and analgesicsThe improved Beitz model was established in 40 male adult SD rats. The animals were randomly divided into control group, zeolite group (Z), zeolite-borneol group (ZB), zeolite-sucralfate group (ZS), zeolite- lidocaine powder group (ZL)(n = 10). After traumatic Surgery,the wound on Left hind limb was used the topical compound medicines above-mentioned. The animals were measured secondary mechanical pain threshold and pain threshold before operation, 1h, 3h, 5h, 24h after operation respectively and compared. Results: It showed that the animals of zeolite-borneol group were significantly higher pain threshold than zeolite control group. the pain threshold of rest groups was no significant difference with control group. It means that the sample of zeolite-borneol compound can effectively control the acute pain (P <0.05). The zeolite-lidocaine powder samples and zeolite-sucralfate samples had no significant analgesic effect.3. The analgesic effect of compound medicines about Ag.Zn-zeolite hemostat and borneolExperimental samples in accordance with the weight ratio of borneol content of 2%, 4%, 8%, 10%, with the corresponding pair of Ag.Zn-zeolite hemostatic agent containing uniformly mixed, made into powder for external use. The improved Beitz model was established in 15 male adult SD rats. The animals were randomly divided into 5 groups: 2%borneol gruop, 4%borneol group, 8%borneol gruop, 10%borneol group, Ag.Zn-zeolite hemostatic agent was addition as control gruop(ZC). After Modeling 4 hours, the animals were sacrificed and removed the lumbar enlargement of spinal cord (L4 ~ L5) which were made of sliced and stained with fluorescent antibody. The results showed that 4% borneol played in the sample the minimum effective analgesic effect of the ratio. |
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