Preparation And Characterization Of Polysaccharide/Zeolite Hemostatic Material

According to the reports, excessive bleeding is the leading cause of death innearly85%of incident, especially when encountered with the severe bleeding woundssuch as artery bleeding. People have nothing to do with this bleeding situation. If itcan be handled promptly, the mortality will be effectively reduced by50%. Therefore,developing a powerful hemostatic material is urgent. Artery typical hemostaticmaterial is mainly polysaccharide, such as chitosan and sodiμm alginate, etc.Polysaccharide is a kind of natural polymer, which used owing to their excellentbiomedical properties, such as anti-inflammatory, antibacterial, wound healingactivity and biodegradability, etc. They show good performance properties and canstop bleeding rapidly. However, due to the limitation of chitosan’s hemostatic effect,chitosan can’t absorb too much water. As a result, blood often leak out from side ofthe wound. Recently, the composite hemostatic material is becoming the hotpot forthe artery hemostat material. Zeolite is the ideal mesoporous hemostatic materials. Ithas excellent hemostatic properties and can stop bleeding within1-5min.The typical methods of preparing organic/inorganic composite material aresol-gel method and blending method. Usually, processing of sol-gel method iscomplex and can’t meet the need of industrialization production. Blending method isused in industry production. Product made in this method, however, is of poordispersion. In our research, the hemostatic material was prepared by blending method.In order to solve existing problem, sodiμm alginate was used as a retention agent,chitosan/zeolite was mixed by blending and frozen-drying method. At the same time,CaCO3/GDL was added as cross-linking agent by situ release method in order to getthe composite sponge with high application performance. This procesure not onlysimplified the preparation process and enhanced the mechanical properties of thematerial, but also improved the dispersion of hemostatic material. Finally, theproducts, with high quality to stop the astray bleeding effectively and accelerate thetime of wound healing, were obtained.Firstly, alginate was blending with zeolite, and alginate/zeolite compositesponge was prepared. Particle size observation, time of particle settlement anddispersion behavior in alginate/zeolite composite sponge was investigated respectively.Secondly, chitosan fibers/alginate/zeolite was mixed together and flexible3D porouschitosan/zeolite composite sponges via the addition of sodiμm alginate were gained.Morphological observation, mechanical property, porosity, swelling ability, and degradation behavior of composite sponges were tested by various physic-chemicalmethods. The results are as follows:(1) Sodium alginate as retention aid exhibits good retention effect. Particle sizedistribution of zeolite is in the range of10-40μm. After zeolite was added intoalginate solution, particle size distribution dramatically increased to10-67.2μm, andtime of settlement was significantly prolonged. When concentration of sodiumalginate is1.5wt%, dispersibility is the best, gelation time of the composite sponge isshortened, and tensile strength of composite sponge is increased. When thecross-linking degree is80%, the tensile strength of composite sponge is up to26.9N.When the composite sponge is60%crosslinking degree, water absorption ratio showsthe maximum value. If composite sponge is encountered with the water, thetemperature was just increased to37.1℃. It is proved that the resulting samples cansignificantly solve the zeolite heat problems.(2) Hemostatic properties of the hemostatic material is tested by the animalexperiments. Self-made hemostatic material was compared with SealFoam, which isthe licensed product of Starch Medical Incorporation’s in the same wound. It is shownthat SealFoam was fragile and easily broken, indicating that its performance propertywas relatively poor. While chitosan fibers/alginate/zeolite composite sponge was softand flexible, it can stop bleeding within2min. Moreover, the blood clot on compositesponge with40%crosslinking degree was observed clearly. The results show thatcomposite sponge can stop astray bleeding effectively. Therefore, the preparedcomposite hemostatic material in our research reveals outstanding hemostatic effect,and it is expected to bring better economic benefits.