Effect Of Kidney Huayu Recipe On The Expression Of Insr Gene And Igfr Gene In Pco Model Rat Ovarian Granulosa Cell Membrane

Objective:By establishing animal model of rat PCO, we used traditional Chinese medicine named Kidney Huayu Recipe to interfere the model, then to discuss the effect of Kidney Huayu Recipe on the expression of INSR gene and IGFR gene in PCO model rat ovarian granulose cell membrane.We wished all this could provide the experiment evidence for clinical therapy of PCOS.Methods:69 cases of female SD rat at 6-week,becoming enamoured having a law periods,were at random divided into six groups, including Group One (model control group) 13 cases,Group Two(high dosages of traditional Chinese medicine group) 14cases,Group Three(middle dosages of traditional Chinese medicine group) 13 cases, Group Four(low dosages of traditional Chinese medicine group) 13 cases, Group Five(Positive Drug control group) 13 cases, Group Six(blank space control group) 13 cases.Group one, two, three, four and five were fed with 0.125 mg/ml Letrozole-Carboxyl methyl cellulose solution,8ml/kg per cases, once a day, last 21 days. Group six according to 8ml/kg per cases were fed with 1% carboxyl methyl cellulose solution,once a day,last 21 days. Afterwar- ds, decocted Kidney Huayu Recipe with water made 2.66 g/ml, low dosages of traditional Chinese medicine group,middle dosages of traditional Chinese medicine group and high dosages of traditional Chinese medicine group were taken 8ml,15ml,30ml diluted to 30ml, 30ml,32ml, fed with lml/150g per cases, (Qd×3 ds, rest 1 d),last 84 days; Positive Drug control group were fed with UK-35,of which were dissolved 1 piece in 64ml distilled water everyday, according to 150ml/g,(Qd×3 ds,rest 1 d),last 84 days, model control group and the blank space control group irrigates were fed with the same volume physiological saline every day. After the end of experimental observation, rats in each group were put to death and drawn.Results:After statistical processing, all of the groups can be detected INSR gene and IGFR gene expressing in the ovarian granulosa cell membrane, in the model control group,INSR gene and IGFR gene expression in significantly lower than blank control group, high dosages of traditional Chinese medicine group, middle dosages of traditional Chinese medicine group,low dosages of traditional Chinese medicine group and Positive Drug control group(P<0.05);blank control group, high dosages of traditional Chinese medicine group,middle dosages of traditional Chinese medicine group,low dosages of traditional Chinese medicine group and Positive Drug control group have no statistically significance among the five set of groups.(P>0.05).Conclusion:These experimental results show that abnormal expression of INSR gene and IGFR gene may be a crucial factor in women with polycystic ovary, by regulating the abnormal expression of INSR gene and IGFR gene,Chinese medicine:Kidney Huayu Recipe play an important role in the treatment of polycystic ovary. Its clinical efficacy is no less than the western medicine commonly used in clinic:Diane-35.