Renal Pathology And Renal Function Effect Of Carbon Dioxide Pneumoperitoneum On The Severe Acute Pancreatitis

Objective:Try to explore CO₂ pneumoperitoneum on Severe acute pancreatitis (SAP) rabbits renal function response and renal pathology.Methods:30 healthy male New Zealand rabbits were randomly divided into 3 groups:(1) CO₂ pneumoperitoneum group,which include 10 rabbits. SAP complicated with renal insufficiency was induced by injection of 5 % sodium taurocholate through retrogradely pancreatic duct via duodenal papilla with epidural catheter,closed puncture hole with medical sealant glue. Then six hours later gave CO₂ pneumoperitoneum at a pressure of 12 mmHg for 120 minutes. (2) Open abdominal cavity group, which include 10 rats.The animals were treated as same as CO₂ pneumoperitoneum group except CO₂ pneumoperitoneu. (3) Control group, sham operation, open abdomen for 30 minutes, and then the abdomen was closed.when gave CO₂ pneumoperitoneum concluded then killed the CO₂ pneumoperitoneum group animals immediately.eight hours after the surgical procedures, the remains were killed .Cut pancreatic and kidney tissue histopathologic examination pathology optical microscope and kindy NF-кB expression index. The blood samples were gathered for examining serum BUN,Cr,NO,ET and AMY. Comparison and analysis of three sets of experimental data.Results:(1) The pathology scores of CO₂ pneumoperitoneum group (10.1±1.64) and the open group (9.3±1.31) were significant difference compare to the control group (0.0±0.00), and have statistical significance (P < 0.05). There was no statistically important differences between CO₂ pneumoperitoneum group and the open group (P = 0.294). The pathology scores of CO₂ pneumoperitoneum group (81.93±6.24) and the open group (73.28±7.33) were significant difference compare to the control group (0.0±0.00), and have statistical significance (P < 0.05). There was no statistically important differences between CO₂ pneumoperitoneum group and the open group (P = 0.285).(2) The concentration of AMY,ET/NO,BUN,Cr and NF-кB expression index in each group were analysised as:The concentration of AMY in serum: CO₂ pneumoperitoneum group: (2856±730); open abdominal cavity group: (2619±693); control group: (422±91). Data in CO₂ pneumoperitoneum group and open abdominal cavity group have statistical significance to control group (P < 0.05); data in CO₂ pneumoperitoneum group has no statistical significance to open abdominal cavity group (P = 0.174).The concentration of ET/NO in serum: CO₂ pneumoperitoneum group: (1.43±0.08); open abdominal cavity group: (1.39±0.06); control group: (0.91±0.03). Data in CO₂ pneumoperitoneum group and open abdominal cavity group have statistical significance to control group (P < 0.05); data in CO₂ pneumoperitoneum group has no statistical significance to open abdominal cavity group (P = 0.312).The concentration of BUN in serum: CO₂ pneumoperitoneum group: (21.63±1.60); open abdominal cavity group: (14.27±2.18); control group: (6.40±1.33). Data in CO₂ pneumoperitoneum group and open abdominal cavity group have statistical significance to control group (P < 0.05); data in CO₂ pneumoperitoneum group also has statistical significance to open abdominal cavity group (P < 0.05).The concentration of Cr in serum: CO₂ pneumoperitoneum group: (214.69±14.67); open abdominal cavity group: (153.66±17.38); control group: (85.17±17.37). Data in CO₂ pneumoperitoneum group and open abdominal cavity group have statistical significance to control group (P < 0.05); data in CO₂ pneumoperitoneum group also has statistical significance to open abdominal cavity group (P < 0.05).The concentration of NF-кB expression index: CO₂ pneumoperitoneum group: (0.3015±0.067); open abdominal cavity group: (0.2898±0.074); control group: (0.1310±0.018). Data in CO₂ pneumoperitoneum group and open abdominal cavity group have statistical significance to control group (P < 0.05); data in CO₂ pneumoperitoneum group has no statistical significance to open abdominal cavity group (P = 0.361).Conclusions:(1) CO₂ pneumoperitoneum probalble not aggravate SAP rabbit's inflammation and pathology of heart sweetbread and kidney.(2) CO₂ pneumoperitoneum probalble not aggravate SAP rabbit's ET/ NO.(3) CO₂ pneumoperitoneum probalble increase the BUN and Cr of the SAP rabbit.