Experiment Study Of The Theory Of "the Lung Associated With The Large Intestine"

1. ObjectivesBased on the theoretical analysis and experimental research of the theory of "The lung associated with the large intestine",the article aims at revealing the rationality of the construction, characteristics and objectivity theory, so as to put forward the evidence of exploiting Xiefei pingchuan ling to treat children phlegm-heat type asthma.2. Methods2.1 Research method of theoriesThe article adopts TCM genetic research method, explaining the formation and evolution of the basic conception,the basic ideas and the basic theory of TCM in lung and large intestine objectively and exactly. Studying the correlation between lung and large intestine according to the conception of these organs in western medicine,Based on the study of the physiological connection of lung and large intestine,to further investigate the relationship between lung diseases and genteropath of their pathogenesis and symptoms.And discuss the material foundation of the theory--"the Lung Associated with the Large Intestine" in traditional Chinese medicine.2.2 Experimental researeh methodSeventy SD rats were equally divided into seven groups randomly:the normal group, the model group, the unhitch group,the Sanao group, the Santong group, the Sanhuo group and the Xiefei pingchuan ling group, Except those in the normal group were untreated, the others rats were established to intestinal obstruction model by incomplete ligation of the rectum in vitro.The ligation was relieved 72h after operation in the unhitch group and the treatment group, and the animals were fed continuously on routine. Meanwhile, Xiefei pingchuan ling and Its decomposed formulas were given.2.3 Determination of Examination target and examination method2.3.1 General condition observation:including big mice'outward appearance,the activity,food eating,,the feces and urine,the temperature,the breath frequency and scope,the respiratory tract secretion,the body weight of each group before and after the model-making.2.3.2 Calculation of lung wet/dry weight ratios:After the animal was killed, choose the upper part of the right lung, weighs it,and then dry it for 48 hours, then weighs it again and calculate the wet/dry weight ratios of the lung.2.3.3 Observation of the pathological change of the lung and large intestine organization:use paraffin section method to entrap the lung and large intestine organization, dye with HE method, and observe with optical microscope.2.3.4 ELISA(enzyme linked immune-sorbent assay) method was used to measure the content of TNF-α,ET;the content of phospholipids and the total proteins(TP) in BALF was evaluated by the methods of determineate phosphorus content and Bradford respectively.3. Conclusions3.1 The theory of "the Lung Associated with the Large Intestine" plays an important role in the clinic treatment.3.2 Xiefei pingchuan ling protect the damaged lung tissue of the model animals by decreasing the level of serum ET,TNF-α,MDA in the lung tissue,protein in the BALF,and increasing the level of TP in the BALF.