The Expression Of β-catenin And DKK-1 In Cervical Squamous Carcinoma And Its Clinical Significance

OBJECTIVETo detect the expression of P-catenin and DKK-1 in chronic cervicitis, CIN,cervical squamous carcinoma,to explore the relationship between the expression of P-catenin or DKK-1 and the genesis,clinic pathological parameters of cervical squamous carcinoma and to explore the function of P-catenin and DKK-1 in the genesis and development of cervical squamous carcinoma.METHODS1.There are 30 patients with chronic cervicitis,30patients with CIN, and 44 patients with FIGO stageⅠb1 toⅡa.The expression of P-catenin and DKK-1 were deteced by immunohistochemistry technique.2.Analysing the relationship between the expression ofβ-catenin or DKK-1 and the age of patients,surgical stage,myometrial invasion,patho-logical grading or lymphatic metastasis; exploring the function ofβ-catenin and DKK-1 in the genesis and development of cervical squamous carcinoma; analysing the correlation betwwen the expression ofβ-catenin and DKK-1.RESULTS1.β-catenin showed nomal expression in 30 chronic cervicitic tissues,and showed abnomal expression(cytoplasic expression) in the tissues of cervical intraepithelial neoplasia and cervical squamous carcinoma. The abnomal expression rates ofβ-catenin in the chronic cervical tissues, CINⅠ,CINⅡ-Ⅲand cervical squmaous careinoma were respectively 0,15.4%,52.9%,79.5%. There was signifieantly dieffrence respectively(P＜0.05). 2.DKK-1 expressed in cytoplasic. The positive expression rates of DKK-1 in the chronic cervical tissues, CINⅠ,CINⅡ-Ⅲand cervical squmaous careinoma were respectively 83.3%,76.9%,35.3%,11.4%. There was signifieantly dieffrence respectively(P<0.05).3. The abnomal expression rate ofβ-catenin and the positive expression rate of DKK-1 were significantly associated with the tumor diefferntiation stuats and high-risk human papillomavirus infection (P<0.05).4.The abnomal expression rate of P-catenin in FIGO stageⅠb1-Ⅰb2 andⅡa were respectively 76.0%,84.2%.There was no signifieantly dieffrence(P<0.05). The abnomal expression rate of P-catenin were not associated with myometrial invasion, the age of patients, pelvic lymph node metastases. (P<0.05)5.The positive expression rate of DKK-1 in FIGO stageⅠb1-Ⅰb2 andⅡa were respectively 16.0%,5.3%.There was also no signifieantly dieffrence (P＜0.05). The positive expression rate of DKK-1 were not associated with myometrial invasion, the age of patients, pelvic lymph node metastases. (P＜0.05)6.There is significant negative correlation between the expression ofβ-catenin and DKK-1.(r=-0.706, P=0.000)Conclusion1.In cervieal squarnous cell malignant transformation process, with the lesions inerease,the abnomal expression rate ofβ-catenin increased, and the positive expression rate of DKK-1 decrese. They may play an important role in carcinogenesis.2. The abnomal expression rate ofβ-catenin and the positive expression rate of DKK-1 were significantly associated with the tumor diefferntiation stuats and high-risk human papillomavirus infection. They may reflect the malignant degree of cervical tissues, and play an important role in carcinogenesis collaborating with high-risk human papillomavirus infection.3. There is significant negative correlation between the expression ofβ-catenin and DKK-1.(r=-0.706, P=0.000) The loss of DKK-1 may promote the abnomal expression of P-catenin, so as to lead to the occurrence and development of cervical cancer.