The Expression And Clinical Significances Of CDK8and β-catenin In Cervical Squamous Cell Carcinoma

BackgroundCervical cancer is originated from the cervical epithelium.In the female reproductive system tumor,cervical cancer has the highest morbidity and mortality. HPV plays an important role in the development of cervical cancer, but not the independent factor. The occurrence and development of cervical cancer is a complex process, many factors involved in. The activation of proto-oncogenes, inactivation of tumor-suppressor genes and abnormal transcription of DNA participate in the occurrence of cervical cancer. CDKs plays an important role in regulating the cell cycle, participates in the occurrence of cancer.CDK8is one of the CDKs. High expression of CDK8is closely related to the occurrence of colon cancer, gastric cancer and melanoma. β-catenin is found as a kind of adhesion factor. There is no study of the expression of CDK8in cervical cancer.β-catenin can mediate adhesion between cells.β-catenin also has the effect of cancer factors.β-catenin is related to the occurrence of colorectal cancer, liver cancer, colorectal cancer, ovarian cancer and cervical cancer.β-catenin is considered participates in the development of the tumor mentioned above。Objective:Detect the expression of CDK8and β-catenin in20cervicitis tissues,64CIN tissues,50cervical squamous cell carcinoma tissues using Powervision immunohistochemical method.Analysis the relationship between the two genes and clinical significances.Results:1. CDK8was not expressed in cervicitis tissues. CDK8was found expressioned in cervical cancer and cervicitis. The expression of CDK8in cervical intraepithelial neoplasia samples was respectively45.8%、55%、90%.The positive rate of CDK8in CINIII and CINII is higher than in CINI (p<0.05). The expression of CDK8was similar in CINII and CINIII(p>0.05). The positive rate of CDK8in cervicitis tissues、 CIN tissues and cervical cancer tissues were respectively10%、62.5%、86%, with a up-regulated tendency. Among the three groups,there was significant difference (P<0.05).2. In normal cells,β-catenin is expressed in the cell membrane.In CIN tissues and cervical cancer tissues, β-catenin was found in cytoplasm and nucleus. The expression of β-catenin in cervical intraepithelial neoplasia samples was respectively16.7%、45%、65%. The positive rate of β-catenin in CINIII and CINII is higher than in CINI (p<0.05). The expression of β-catenin was similar in CINII and CINIII(p>0.05). The positive rate of β-catenin in cervicitis tissues、 CIN tissues and cervical cancer tissues were respectively0%、40.6%、70%, with a up-regulated tendency. Among the three groups,there was significant difference (P<0.05).3. In cervical cancer,the expression of CDK8in node-positive cases were significantly higher than node-negative cases (P<0.05),whereas no relations to clinical stage and histological differentiation(p>0.05).4. In cervical cancer,the expression of β-catenin in low differentiation and node-positive cases were significantly higher than high differentiation and node-negative cases (P<0.05), whereas no relations to clinical stage.5. There was significant positive correlation between the expression of CDK8and β-catenin in cervical cancer(r=0.365).Conclusion:1. From cervicitis tissues to CIN tissues to cervical cancer tissues the expression of CDK8and β-catenin were increased. Both two may promote the occurrence of cervical cancer2. The expression of CDK8in cervical cancers was related to lymph node metastases.The expression of β-catenin was related to histological differentiation and lymph node metastases.The expression of CDK8and β-catenin may work together to promote the metastasis of cervical cancer.3. The expression of CDK8protein is related to the β-catenin protein in cervical lesions, they were positive correlation. They may play important roles in the development of cervical cancer.