Metabonomics Study Of Serum And Urine On The Model Of Abnormal Savda Disease Carring Diabetes Mellitus By NMR

Objective: based on the establishment of Abnormal savda disease rat model with Diabetes Mellitus,appling the metabonomics technology through nuclear magnetic resonance (NMR),, analyse and discover the biological marker by Metabonomics study of Serum and urine of Abnormal savda model group,diabetes Mellitus model group, Abnormal savda disease carring Diabetes Mellitus model group and control group,investigate the substance metabolism network of Abnormal savda disease earring Diabetes Mellitus, explore the Pathophysiological background and function in the process of Abnormal savda disease carring Diabetes Mellitus.Methods: According to the theory of Uighur traditional medicine, Abnormal savda disease carring Diabetes Mellitus rat model was established, A 1H NMR based metabonomic analysis was performed on these serum and urine samples. A multi-steps date preprocessing procedure was completed and then Orthogonal Partial Least-Squares Discriminant Analysis(OPLS-DA) was fulfilled to distinguish the metabolic phenotypes, the influential biomarkers accounting for the metabolic signature perturbation were discovered by Pearson's product moment correlation coefficient and 2D NMR.Results: It was found that between the H NMserum 5'-nucleotidase of four group exist signification difference, the variability of metabolin in the serum of four group were VLDL, LDL, unsaturated fatty acid, acetone, acetoacetic acid, pyruvic acid, citric acid, glycine, alanine, tyrosine, Phe acid, leucine, isoleucine, citrulline, methionine, creatine, inositol, shark inositol, urea,etc. (P<0.05), the variability of metabolin in the urine of four group were citric acid, guanidine acetic acid, tyrosine, methionine, lysine, phenylalanine, tryptophan, isoleucine, valine, alanine, aspartic acid, creatinine, etc. (P<0.05).Conclusion: the metablolite in the serum and urine of those Abnormal savda, diabetes, Abnormal savda disease carring Diabetes Mellitus and control group were significant differences.the development of abnormal savda can cause the metabolism disorganization of lipid.the glycolysis and energy synthesis metabolisim disorder in the Diabetes group.glycometabolic disturbance, creatine dyssynthesis are the congenerous Pathophysiological process of Abnormal savda and diabetes group.the sianificant difference of the variability of metabolin in the urine is more than the sianificant difference of the variability of metabolin in the serum.