| Objective: Chronic atrophic gastritis with mild dysplasia is more common clinical disease, the incidence rate in the forefront of a variety of stomach, cancer rate about one per cent per year, and intestine metaplasia is recognized as precancerous lesions. Its effective treatment is the key to prevention and treatment of gastric cancer, Because of their complex etiology and pathogenesis, potential lingering illness, and easy to cancer, the current Western medicine mainly endoscopic follow-up, treatment is limited to symptomatic treatment, therefore, expand the group of developing serious side, a better prescription great clinical value. YingLianjin Huazhuo detoxification Fang because the Treatment of chronic atrophic gastritis made a good clinical effect, it attempts to test the animal from different perspectives of the role of the therapeutic mechanism of chronic atrophic gastritis, which YingLianjin Huazhuo detoxification Fang in clinical applications experimental basis for a more detailed and objective to broaden the therapeutic areas to explore and research new treatment methods and effective drugs.Methods: 90 Wister rats were randomly divided into normal group, model group, the YingLianjin Huazhuo detoxification Fang with large, medium and low-dose group and the group Weifuchun 6 groups. Other than the normal group while the remaining five groups were integrated modeling method: 20mmol / L of sodium deoxycholate 2ml daily fasting stomach; 0.02% ammonia solution, free drink for animals, with irregular diet method, the first 5 Week libitum water, 5 weeks after a single day of fasting, free access to water, two water at libitum. Criterion-referenced successful model of Chinese Medical Association in 2003 Digestive Endoscopy credits will be to develop a "chronic gastritis endoscopic grading criteria and treatment of type comments into the trial." After the modeling, the model group to take the necessary pathology and blood samples were frozen after pretreatment for the detection of relevant indicators. The remaining five groups on the second day of drug treatment: large dose group of Chinese crude drugs according to 96g / kg orally 1 time a day, medium-dose group received traditional Chinese medicines by 64g crude drug dose / kg orally 1 time a day, low-dose group received 32g Chinese crude drug / 1 kg per day orally, Weifuchun group fed 1 time a day at 144mg/ml, the normal group and model group fed the same volume of normal saline per day, each group intragastrically for 1 month. After the last administration, fasting for 24 hours decapitated blood, each blood 4ml, and immediately split the stomach drawn along the gastric greater curvature longitudinal incision, the stomach tissue fixation, dehydration, embedding, slicing, etc. HE staining, pathological examination and the microscope camera. Observe the general condition of rats, gastric mucosal histological changes, protein was determined by immunohistochemistry of epidermal growth factor (VEGF) and cyclooxygenase 2 (COX-2) levels, determined by ELISA in serum pepsinogen ( PGI) levels, and application of SPSS 13.0 statistical software for statistical analysis.Results: (1) General situation: After treatment, the rats fur modeling, mental state, diet, activities were significantly improved, especially in Britain and YingLianjin Huazhuo detoxification Fang large, medium dose group showed the most obvious. YingLianjin Huazhuo detoxification Fang weight of large and medium dose group than Weifuchun control group, there was significant difference (P <0.05). (2) gastric histopathological observation: high dose group scattered in the old bleeding points, folds horizon, scarce, most close to the normal group, the gastric mucosa clearly structured arrangement of regular mucosa, gastric glands and mucous cells .cells similar to the normal control group, no significant intestinal metaplasia and mild dysplasia, better than Weifuchun group. That even the British can only antidote was to eliminate Huazhuo gastric inflammation, improve or even reverse the chronic atrophic gastritis with mild pathological changes in the stomach Dys. (3) PGI: PGI large-dose group were expressed as an average of 78ng/ml, the medium dose of PGI rats expressed as 71ng/ml, low-dose group were expressed as mean PGI 58ng/ml, rats Weifuchun expressed as mean PGI 50 ng / ml. large and medium dose group was significantly higher than Weifuchun group (P <0.05). (4) VEGF: high-dose group 40% of the average positive rate of VEGF, in the dose group 49% of the average positive rate of VEGF, VEGF-positive low-dose group 60%, Weifuchun average VEGF-positive rate of rats 66%. large and medium dose group was significantly lower than Weifuchun group (P <0.05). (5) COX-2: high-dose group an average of COX-2 positive rate of 38%, medium dose group an average of COX-2 positive rate of 42%, low-dose group were COX-2 positive rate of 61%, stomach complex spring of rats COX-2 positive rate on average 69%. large and medium dose group was significantly lower than Weifuchun group (P <0.05).Conclusions: The study looked at the YingLianjin Huazhuo detoxification Fang treatment efficacy of chronic atrophic gastritis with mild dysplasia , discussed the YingLianjin Huazhuo detoxification Fang mechanisms, which may be (1) to increase blood flow, improve microcirculation, regulation of immunity, so that reversal of abnormal cells to normal cells. (2) the elimination of pathogens causing stomach and promote en secretion of normal glands, improving digestion(3) reduce over-expression of COX-2, and then to inhibit the epidermal growth factor receptor pathway, reduced VEGF expression in gastric cancer cells, increased PGI levels for therapeutic purposes. This experiment proved that even the gold Huazhuo British Treatment of detoxification treatment of chronic atrophic gastritis with mild dysplasia valid prescription, can significantly improve symptoms and reverse the glands atrophy and dysplasia. Its efficacy is superior to Weifuchun group, for the prevention and treatment of precancerous lesions of gastric cancer provides a new idea to rich the theoretical system of Chinese medicine drug cloud. |
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