Selection Of Evaluation Markers By Absorbed Components

Based on the hypothesis of"the ingredients in complex prescription >> absorption ingredients > pharmacological ingredients", the absorption ingredients of the extract of TCM icluding Paeoniae Radix Alba,Paeonia Veitchii Lynch and Plantaginis Semen, were compared by the models in vivo and in vitro-everted intestinal sac (VEIS), respectively. The feasibility of the model of in vitro were evaluated and a rapid method of selection of evaluation markers by absorption components (SEMAC) was established.In order to find the rapid method of the absorption components, the intestinal absorption of Paeoniae Radix Alba were investigated with a model in vitro-everted intestinal sac. The results showed that four absorption ingredients in blood can be detected by HPLC in the intestinal bag fluid, the corresponding relations between two models were found. SEMAC could be used as a tool to find the absorption ingredients of Paeoniae Radix Alba. The detecting the 60min - 90min samples after administration in vitro model is suggested which may be absorbed into the blood".The results showed that the absorption ingredients of three concentration of Paeonia Veitchii Lynch were investigated with the VEIS model. The intestinal sac fluids of jejunum and ileum were collected in different time and detected by HPLC. The accumulative absorption quantity of albiflorin, paeoniflorin were calculated, respectively. Five ingredients could be detected. In different concentrations of P. Veitchii Lynch, albiflorin and paeoniflorin in various intestines section were the linear absorption (R~2> 0.9), conformed to the zero order absorption rate .The values of Ka in the jejunum and ileum were increased along with the raised dosage of the P. Veitchii Lynch (P﹤0.05), indicating a passive absorption manner. SEMAC could be used as a tool to find the absorption ingredients of P. Veitchii Lynch. Comparing with the jejunum, the ileum could provide the more absorption information. It was showed that the detecting was 60min - 90min.The absorption of three concentrations of Plantaginis semem was investigated with the VEIS model. The intestinal sac contens of jejunum and ileum were collected at different time and geniposidic acid was detected by HPLC and LC-MS~n as the representative marker. Total six ingredients could be detected. At different concentrations Plantaginis semen, geniposidic acid tested by VEIS showed that there was a good linear correlation between the drug absorption from the medium across the intestinal epithelium into the sac contents in various intestines section. The absorption of the gut sacs from 0 to 90 min manifested a significant time-dependent manner. The Ka of geniposidic acid in the jejunum and ileum was increased along with the raised dosage of the Plantaginis semen (P﹤0.05), which indicated a passive absorption manner. This method can be used as a tool to investigate the absorption ingredients of Plantaginis semen. Comparing with the jejunum, the ileum can provide the more absorption information fast. The optimal incubation time in intestinal sac was 90 minutes.On the basis of the above results, the SOP of SEMAC was constituted and used as a guiding principles for choosing the quality evaluation of TCM.