Experimental Studies On Tongxieyaofang Medicine By Semi-bionic Extraction (SBE)

Objective:To optimize the conditions of Tongxieyaofang Medicine by semi-bionic extraction (SBE). To study the quality control of Tongxieyaofang Medicine.Method:First, we optimize the best extraction conditions of the semi-bionic extraction for Tongxieyaofang Medicine with peoniflorin, prim-o-glucosylcimifugin, hesperidin, 5-O-methylvisamminol hilieidum,the characteristic peak area of Atractylodes macrocephala, the total integral area by HPLC, dried extract weight as the indexes. Then, with the indexes mentioned above, we optimize the best alcohol precipitation concentration. According to the former conditions, the four kinds of extract methods, including the semi-bionic extraction(SBE), the water extraction (WE), the semi-bionic extraction by precipitation with alcohol (SBAE) and the water extraction by precipitation with alcohol (WAE) were compared by the contents of the index ingredients.Furthermore we study the quality control of Tongxieyaofang Medicine.Result:The technological conditions of the semi-bionic extraction for Tongxieyaofang Medicine is pH in turn:2.0,6.5,8.5; Time:60min,30min,30min. the best alcohol precipitation concentration is 70%. The sequence of total index Y value of the four extraction technology is:YSBE> YWE >YSBAE>YWAE.The HPLC fingerprint of Tongxieyaofang Medicine have 13 peak.And anyone of 4 medicinal herb has its characteristic peak.Conclusion:SBE method is the best among the four methods in the extraction of components of Tongxieyaofang Medicine. It is stable and reliable that HPLC fingerprint of Tongxieyaofang Medicine.And we can use the HPLC fingerprint to control the quality of Tongxieyaofang Medicine.