| Background and Objective Gastric cancer is one of the most common gastrointestinal malignancy which serious harm to human health. Although the incidence of gastric cancer decreased gradually in the world, it's still ranked the 4th common cancer, the mortality rate remains high, accouting for No.2 cancer. The mainly reason is the low rate of early diagnosis(I state is only for 10%), surgical resection is low(radical excision is only for 50% to 65%),5-years survival rate low(overall survival rate are only about 30%).Complete surgical removal of the foci of gastric cancer is still the most radical means, but the cancer recurrence rate is as high as 50% to 70%,5-years survival rate is only for 20% to 50%.In order to improve the prognosis of patients with gastric cancer people have been seeking ways to treat gastric cancer other than surgery. Since 1960s, the chemotherapy began to utilization in gastric cancer ,from then on, the chemotherapeutics and programs were come out one after the other. But the chemotherapy don't work well in gastric cancer due to the low sensitivity and the heterogeneity of the gastric cancer cells. So there is a big difference effect of chemotherapy between the individual, it is difficult to acquire satisfactory results. Therefore, from the choice of chemotherapy,combination dosage and usage,people upgrade and try to expect to improve the efficacy of chemotherapy and reduced toxic and side-effects uninterrupt in recent years.These become one of the focuses in the chemotherapy in gastric cancer. The purpose of our study is aimed at the outstanding problems of the chemotherapy in gastric cancer now. We detection the sensitive of the 11 kinds of common chemotherapeutic drugs on human gastric cancer cell strain MGC-803 by MTT assay.In order to screen more sensitive drugs.To study the inhibitory action of the different order administration on gastric cancer cell strain MGC-803 and to explore the possible best sequential chemotherapy with sensitive drug. Expecting provide the basis trial in order to improve the chemotherapy therapeutic effect in clinical.Method The human gastric cancer cell strain MGC-803 was inoculated with 10% FBS( Fetal Bovine Serum) RPMI-1640 and cultivated in conventional culture way. 1. Proliferation curves drawing: MTT analysis were used on the detection of proliferation of the cell strain MGC-803 and standard cell proliferation curves of different denstity were made. Appropriate cell concentration was selected as the initial inoculation denstity according to the concentration of cell growth.2.Screening test of sensitive drug: Detect the inhibitory action of 11 different chemotherapy drugs on the cell strain MGC-803 in different concentrations and different time points(24h,48h,72h). To screen the sensitive drug and draw out the time-effect, dose-response curve of the inhibitory action of sensitive drugs on cell strain MGC-803.3.Sequential treatment of sensitive drug: According to the results of the screening test of the previous step, we chose the concentration of the sensitive drug which controlling the tumor cell inhibition ratio at 20% to 25% on the 24h and undertake the sequential administration test(the administration of DDP concentration is 50%TDC and DOC concentration is 6.25%TDC ).Results 1.Observing the appearance of the tumor cells by inverted phase contrast microscope: part of sensitive drugs have significant inhibitory action on the cell strain MGC-803.The number and morphology of tumor cells changed with the concentration and time.2.The growth curve of the MGC-803 shows that OD value was gradually increased with increasing concentration and incubation time. The cells shows a good linear relationship at the initial density of 1×104-4×104/ml within 72h.The study selected 2×104/ml as the initial inoculation density.3.The sensitivity of different chemotherapy drugs on human gastric cancer cell MGC-803 have significant different. The sensitivity of the order from high to low of the 11 chemotherapy drugs are DOC, PTX, 5-Fu, DDP, VDS, EPI, L-OHP,ADM,MMC,VCR and VP-16. 4.The MGC-803 cell growth inhibition rates in DDP group, DOC group, DDP+DOC group, DDP→DOC group and DOC→DDP group are 27.12%,30.06%,31.32%,30.26%,36.14%.The strongest inhibition on the MGC-803 is DOC→DDP group ,the difference has statistical significant compared with other 4 dosage regimen(P<0.05).But there's no difference between DDP→DOC group and DDP+DOC group dosage regimen ethier in two single-agent group.Conclusion 1. There're different sensitivities on different chemotherapy drugs on human gastric cancer cell MGC-803.2.DOC,PTX,5-Fu and DDP alone have strong inhibitory effect on the cell MGC-803.3.To give DOC before DDP may be the best sequential administration in inhibition of cell proliferation.4.The choices,combinations,doses and orders in sequential chemotherapy for gastric cancer treatment are worthy of further study. |
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