The Study Of Effects Of Mesenchymal Stem Cells From Human Gastric Cancer On Proliferation And Invasion Of Human Gastric Cancer Cell Strain SGC-7901

Effects of mesenchymal stem cells from human gastric cancer on proliferation and invasion of human gastric cancer cell strain SGC-7901Objective To investigate effects of the mesenchymal stem cells isolated and cultured from human gastric cancer on proliferation and invasion of human gastric cancer cell strain SGC-7901. Methods The condition medium of hGC-MSCs (MSC-CM) was isolated, MSC-CM was applied on SGC-7901 cells at different concentrations (20%,40% and 60%), the changes of proliferation and invasion were tested by methylthiazol tetrazolium (MTT) and Transwell chambers respectively; the expressions of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinases 9 (MMP-9) were detected by Real-time polymerase chain reaction (PCR), Western blot and Immunocytochemistry.Results MTT results showed that the proliferation of SGC-7901 was significantly inhibited by MSC-CM and the inhibition rate was positively correlated with MSC-CM concentration and reaction time (P<0.05). The transwell chambers results revealed that cell invasion was significantly decreased after cells were dealed with the MSC-CM, penetrating cells in the control group and the MSC-CM groups at different concentrations (20%,40%,60%) were 75.71+7.32,63.68±7.12,54.29+6.97 and 41.36±3.29 (P<0.05), respectively. Real-time PCR results showed that MSC-CM could down-regulate the expression of PCNA and MMP-9 (P<0.01). Western blot revealed the expression of PCNA in MSC-CM groups at different concentrations (20%,40%,60%) were down-regulated to (73.99+16.71)%, (51.56+12.18)%and (40.11±9.37)%, MMP-9 (74.35±19.12)%, (3.59±10.41)% and (29.58±7.93)%, Immunocytochemistry revealed the average absorbance of PCNA in MSC-CM groups at different concentrations (20%,40%,60%) were 0.201±0.049,0.185±0.031,0.154 ±0.019 and 0.128±0.012, MMP-9 were 0.182±0.032,0.164±0.028,0.136±0.017 and 0.098±0.008 (all P<0.05).Conclusion Proliferation and invasion of SGC-7901 were inhibited by hGC-MSCs and the mechanism may be related to the reduction of PCNA and MMP-9 expression.