The Clinical Study Of PenQiangYan Granule On Treating Chronic Pelvic Inflammatory Disease And The Effect On Inflammatory Related Immune Factors Of The Experimental Model Rats

Objective:To explore the immunoregulation mechanism of the treatment of PenQiangyan Granule on the rats with chronic pelvic inflammatory disease(CPID) by observing the clinic study of Granule on treating chronic pelvic inflammatory Disease (CPID)of blood stagnate and deficiency of the kidney and the changes of inflammatory cytokines and neuclear factor expression in serum or uterine tissue of CPID model rats,and provide evidences for its clinical application.Method:60 cases of chronic pelvic inflammatory disease were divided into two groups randomly:Pen Qiangyan Granule treatment group and Pen Yanjing Granule control group, each group of 30 examples. Female Wistar rats were used and the CPID model was established with mixed bacteria injection combined with mechanic injury. Sixty-five rats were randomly divided into seven groups. After the histomorphological changes of the rats' uteri were observed,we assayed the concentration of pro-inflammatory cytokines IL-8 and anti-inflammatory cytokines IL-10 in serum of rats in each group and detected the expression of the following indices in rats' uterine tissue:neuclear factor-kappaB(NF-κB) and inhibitor of nuclear factor-kappaB(IκB).Result:The total effective rate and the recovery rate of the clinical curative effect and traditional Chinese medicine symptom curative effect of the treatment group and the control group are significantly different(P<0.01), stating that the treat group's curative effect is superior to contrasting group.After treatment by Pen Qiangyan Granule, two groups of medicines can imporve patinet's comprehensive points,traditional Chinese medicine symptom points and some body to solicit points, there are a significant difference(P<0.05), state that the treat group's curative effect is superior to contrasting group.The serous levels of pro-inflammatory cytokines (IL-8) level in model group are significantly higher than those in normal group(P<0.01);the serous levels of anti-inflammatory cytokines (IL-10) in model group are significantly lower than those in normal group. The expression of NF-κB and IκB are all significantly increased than those in normal group. After treatment, the pro-inflammatory cytokine levels are decreased while the anti-inflammatory cytokine levels are elevated. The expression of neuclear factor-kappaB(NF-κB) and inhibitor of nuclear factor-kappaB(IκB) in the uterine tissue of model group is significantly decreased(P<0.01).Conclusions:Pen Qiangyan Granule has effective clinical result in the treatment of Chronic Pelvic Inflammatory Disease with blood stasis asthenia of kidney. Pen Qiangyan Granule could adjust levels of the inflammatory cytokines and neuclear factor expression, thus restore the immune balance effectively so as to inhibit inflammation. The mechanism might be that Pen Qiangyan Granule could regulate the levels of inflammatory cytokines and the activation of NF-κB,and relief Inflammatory reaction.