Study On The Expression Of VEGF,p-Akt And Survivin And Cell Apoptosisin Lichen Planus

Objective:To investigate the expression of VEGF,p-Akt and Survivin proteins and the level of apoptosis of keratinocytes. Analyse the role of PI3K/Akt signal pathway in the pathogenesis of lichen planus.Methods:Immunohistochemical technique was employed to study the expression of VEGF,p-Akt and Survivin in 30 cases of lichen planus and 30 cases of normal skin.TUNEL was used to study the apoptosis of keratinocytes.The relationship among the expression of VEGF,p-Akt and Survivin was analyzed.Results:The positive expression of VEGF,p-Akt and Survivin shows yellow or brown granules in the cytoplasm,the IOD(integrated optical density) values to the strength of the positive expression.Compared with the normal group,the IOD of VEGF,p-Akt and Survivin were 13.584±1.131,14.405±1.263 and 10.537±1.212 which were upregulated in lichen planus speciens(P< 0.01), and there was significantly positive correlation between the expression of VEGF and Survivin, VEGF and p-Akt, p-Akt and Survivin in lesional epidermis(P <0.01).The keratinoytes AI was 72.817±9.234 in lichen planus, which was significantly higher than its 28.160±3.464 in normal skin(P<0.01).Conclusion:The expression of surviving can be promoted by the high level expression of VEGF via PI3K/Akt signal pathway in lichen planus.The pathogenesis of Lichen planus may be associated with the PI3K/Akt signal pathway. The hyperproliferation and overapoptosis of keratinocytes may coexist.