The Expression Levels Of AQP-1 And VEGF In Rat Retinal Ischemia/reperfusion Injury

Objective To investigate the expression levels and significance of AQP-1 and VEGF in the retinal ischemia/reperfusion injury.Method The model of transient ischemia/reperfusion injury of the rat retina was constructed by elevation of the intraocular pressure.The expression levels of AQP-1 and VEGF was measured at 6 hour,12 hour,24 hour,48 hour,3 days,7 days of reperfusion after retinal ischemia by using immunohistochemical staining. Normal retina was treated as control group.Results VEGF positive cells were rarely found in normal group and began to express after 12 hours in ischemia group, increased gradually and reached maximal levels at 48 hours after RIRI (0.523±0.101). There was statistical difference between 48 hours group and other ischemia/reperfusion group (P< 0.01). AQP-1 positive cells were found in the outer retina in normal group, increased gradually in ischemia/reperfusion group and also were found in the inner retina at 7 day after RIRI, and the difference was significant (P< 0.01).Conclusions AQP-1 and VEGF may play an important role in various blinding ischemic ocular diseases, and co-effected the water metabolism of retina.