Experimental Research Of Behavior Change And Cytokines Content About Acupuncture On Insomnia Rat Model Of Sleep Deprivation In Different Periods

Objective:To observe the effect on the behavior and immune cytokine of sleep deprived mice with acupuncture in the experiment and to explore the mechanism of insomnia mice for the purpose of providing some experimental proof about acupuncture therapy on insomnia.Methods:Eighty male SD mice were randomly divided into control group(CC group), model group(S group), insomnia with western medicine(SX group), insomnia with acupuncture(SZ group). Except control group, other groups were divided into three groups according to the different time of sleep deprived:24 hours,72 hours and 120 hours. Each group had eight mice. Duplicate insomnia model on rats by means of water atmosphere. Take acupuncture Shenmai, Zhaohai, Shenmen and SIShengCong as theory methods on SZ group while dixipan zhusheye was taken by SX group. Select the content of IL-1β,IL-2,IL-6,TNF-a in the eye of mice as observing standards. The clinical effect and the influence on open field test and behavior symptom were studied.Results:①Behavior change:In the behavior test, the symptom of acupuncture group (SZ group) was significantly less than that in the control group (P<0.05), and the time of acupuncture therapy for 120 hours was significantly better than other time (P<0.01).②Changes of cytokines levels In the test of immune cytokine, IL-1βand TNF-aof acupuncture group for 72 hours and 120 hours (SZ group) was significantly more than that in the control group (P<0.01,P<0.05), and IL-6 for 120 hours in acupuncture group(SZ group) was significantly more than the control group (P<0.01). Compared with the western medicine group(SX group), the acupuncture groups(SZ group) in different time was better than it significantly in immune cytokine.Conclusions:①Water atmosphere is the effective method for deprive sleep of mice and make insomnia model.②Acupuncture Shenmai, Zhaohai, Shenmen and SIShengCong has improved behavior effects as reaction and learning ability.③Acupuncture Shenmai, Zhaohai, Shenmen and SIShengCong has sedative and hypnotic effects with extended time of therapy.④Acupuncture Shenmai, Zhaohai, Shenmen and SIShengCong has increased the IL-1β,IL-2,IL-6 and TNF-asedative and decreased the damage of insomnia.