Microwave-assisted Synthesis And The Antioxidant And Immune Function Of Glucooligosaccharides

In this paper, the synthesis conditions of microwave solid-phase synthesize glucooligosaccharides were optimized and the antioxidant activity in vitro, the regulatory function of the glucooligosaccharides on the oxidative stress and inflammatory response were studied. Finally, we evaluated the food safety of the products and its effect on antioxidant system of the mice fed high-dose glucooligosaccharides.Glucooligosaccharides were produced by tunnel microwave solid-state reaction equipment. The reaction conditions were optimized by three-factor-three-level general rotation design. Three glucooligosaccharides with different degrees of polymerization were selected to study the scavenging activity to free radicals in vitro and the regulatory function on the oxidative stress and inflammatory response.Kunming mice consumed an ordinary diet were filled the stomach saline, FOS, LGOS, MGOS,HGOS respectively every day. After 21 days, estabilishing the model mice of the oxidative stress and inflammatory response under LPS induce to detect antioxidant indicators(ROS,GSH-Px,CAT,T-AOC,MDA)and cytokines indicators(IL-1,TNF-α,NO,NOS). We evaluated the food safety of the glucooligosaccharides and its effect on antioxidant system of the mice fed high-dose (2.0%, 4.0%, and 8.0% of adding volume) glucooligosaccharides by acute toxicity test and 30-days feeding test.The results were as follows: (1) The final optimized reaction conditions of the quality of glucose substrate was 3.0 kg was build as 28.3 min of microwave heating time, 4.4% catalyst amount, 19.6% initiator.The highest conversion rate of product was 80.1%. (2) Three glucooligosaccharides with different degrees of polymerization had scavenging activity to free radicals in vitro and in vivo. The glucooligosaccharides could efficiently reduce the free radical of mice filled the stomach 0.5g/kg·bw glucooligosaccharides with different degrees of polymerization in blood and liver. Activities of antioxidant activity (GSH-Px, CAT) and T-AOC in in plasma and liver were significantly increased and MDA was significantly decreased. The glucooligosaccharides also can reduce the content of cytokines (IL-1, TNF-α, NO) and the activity of iNOS. Correlation analysis showed that oligosaccharides may regulate the oxidative stress and inflammatory response by inhibiting the generation of free radicals and enhance antioxidant enzyme activity.(3) The acute toxicity LD50 of glucooligosaccharides was greater than 15 g /kg·bw, so it is non-toxic level.The antioxidant activities of the different-dose groups were higher than in the control group. In the kidney, T-AOC of the high-dose group has a significant reduction than the control group and MDA has a significant exaltation. The high-dose glucooligosaccharides (8.0%) could lead scavenging activity of the kidney to reduce.