| Objective: To study the expressions of heat shock protein 27(HSP27) in gerbil's brain tissue and HSP27 antibody in serum following cerebral ischemic reperfusion in gerbils and the effects of flunarizine and aspirin on them.Methods: A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids. Seventy gerbils were randomly divided into: normal control group, sham operation group, ischemia-reperfusion(IR) group, flunarizine intervention group and aspirin intervention group. Gerbils were fed with flunarizine(20mg/ kg) the day before experiment in flunarizine intervention group while aspirin intervention group were fed with aspirin(5mg/kg) twice a day for five days. At 1d, 3d, 7d and 14d after IR , the expressions of HSP27 in brain tissue were detected by using immunohistochemistry technique and the serum HSP27 antibody was detected by means of enzyme-linked immunosorbent assay.Results:1.Compared with control group and sham operation group , ischemia-reperfusion (I/R) group showed severe damage such as neurons degeneration, necrosis , hyperplasia of gliocyte , which were most obviously seen in 7d subgroup. Histologic damage induced by I/R was significantly ameliorated by treatment with flunarizine or aspirin.2. There were few HSP27 positive neurons and gliacytes in control group and sham operation group, which shows no significant difference(P >0.05). Compared with normal control group and sham operation group, HSP27 expressions in neurons and gliacytes were evidently increased since the 1st day following IR, and reaching a peak at the 7th day. There still has a strong expression of HSP27 even 14th days following IR.3. Compared with IR group, the HSP27 expression in flunarizine and aspirin intervention group were evidently decreased at the 1st day, 3rd day, 7th day and 14th day ( p < 0.05 )4 Compared with normal control group and sham operation group, serum HSP27 antibody increased since the 7th day (P<0.05) and reached to a even higher level at the 14th day following IR (P<0.01).5 Compared with IR group, serum HSP27 antibody in flunarizine intervention group and aspirin intervention group were significant decreased at the 7th day and 14th day ( p < 0.05 )Conclusions:1. The Level of serum HSP27 antibody increased following cerebral ischemia-reperfusion and may serve as an important indicater to monitor the damage of the brain tissue following IR.2 Expressions of HSP27 protein increased following cerebral ischemia-reperfusion, which may be a protective factor to the brain tissue.3. Flunarizine and aspirin can significantly protect neurons against cerebral ischemia, regulating the expression of Hsp27 may be involved in mechanisms of treatment. |
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