Establishing Methods And Evaluations Of Experimental Stress Fracture Model In Rabbits

With the increasing intensity and subjects of military training, military training injuries are becoming more and more prevalent, which can depress the combat effectiveness severely. Especially, stress fractures are usually treated as the representative index of MTI for their high prevalence rate, excruciating pain and difficulty to be diagnosed by routine methods.During military training, stress fractures occurred in tibias take up a great proportion, which are high than 50%. Routine X-ray examination can't discover the early sign of stress fractures, while skeletal scintigraphy is much sensitive to early stress fractures, and is regarded as gold standard of diagnosis.Stress fractures result from multiple factors, whose pathogenesis has not been identified. A reliable animal model is necessary for further studies on stress fractures.By developing an exercise system and carrying out related animal experiments, we established a stress fracture animal model, and made a preliminary progress on relationship between exercise intensity and stress fracture degrees.Major contents can be summarized as follows:1. Designed rabbit exercise experiment system based on MSP430 MCU, which can generate high voltage pulses to force rabbits to run and jump.2. Established an Exercise-induced stress fracture model in rabbits by using the exercise experiment system.3. Evaluated stress fractures degrees in rabbit tibias through skeletal scintigraphy.4 Through statistic methods, we analyzed effects exercise intensity contributes to stress fracture degrees.Conclusions can be categorized as follows:1. The rabbit exercise experiment system can adjust exercise intensity and quantity quantitatively with high reliability and expediently.2. The rabbit exercise experiment system and skeletal scintigraphy can be reliable to establish animal mode successfully in short time. On the exercise intensity degree of 10 times per minute and 400 times per day, exercise-induced stress fracture model in rabbits can be established in 1 to 2 weeks.3. Exercise frequency and quantity are positively correlated with prevalence rate of stress fractures in rabbit tibias, among which, exercise frequency plays a much more important role. So we can reach the conclusion that lower training frequency during military training courses may result in lower prevalence rate of military training injuries.