The Research Of The Renal Injury Caused By High Intensity Military Training

Partâ… :Establishment of the animal models with renal injury caused by high intensity military trainingObjective To establish rat models with acute and chronic renal injury caused by high intensity military training that could be used for investigation. Methodsâ‘ One-time exhausting training:Forty male SD rats,12 weeks old, were randomized into control group(n=8), common-training group(n=16)and military-training group(n=16)with the latter two groups taking on one-time exhausting running exercises on treadmill by different ways. Observe the different values in serum, urine and tissue right after training and 24 hours later.â‘¡Continue training:Thirty male SD rats,4 weeks old, were randomized into control group(n=10), common-training group(n=10)and military-training group(n=10)with the latter two groups taking on continue moderate to high intensity running exercises on treadmill by different ways for 8 weeks. Observe the different values during different time.Resultsâ‘ During the one-time exhausting training,compared with the control group and the common-traing group,the urine protein, urinary albumin, urease,bun,scr,ua,and angiotoninâ…¡of the military-training group were increased obviously right after traing (P<0.05).24 hours later,the values of the last group were still higher than the other two(P<0.05), and pathological changes appeared.â‘¡During the continue training, compared with the control group and the common-traing group,the urine protein, urinary albumin, urease,bun,scr,ua,and angiotoninâ…¡of the military-training group were increased obviously (P<0.05).Conclusions The two models are practicable,reasonable and repeatable for investigating the acute and chronic renal injury caused by high strength military training. Partâ…¡The research of the pathogenesis about renal injury caused by high intensity military trainingFirst The changes of activities about Na~+,K~+-ATP ase in kidney during high intensity military trainingObjective To study the changes of activities about Na~+,K~+-ATP ase in kidney during the high intensity military training. Methodsâ‘ One-time exhausting training:Twenty four male SD rats,12 weeks old, were randomized into control group(n=8), military-training group(n=16)with the latter taking on one-time exhausting running exercises on treadmill according to the animal model. Observe the different values of urine and the activities of Na~+,K~+-ATP ase in kidney right after training and 24 hours later.â‘¡Continue training:Forty male SD rats,4 weeks old, were randomized into control group(n=20)and military-training group(n=20)with the latter taking on continue moderate to high intensity running exercises on treadmill according to the animal model for 8 weeks. Observe the different values at 4 weeks and 8 weeks.Resultsâ‘ During the one-time exhausting training,compared with the control group,the urine protein, urease of the military-training group were increased obviously right after traing,while the activities of Na~+,K~+-ATP ase in kidney were decreased obviously(P<0.05).24 hours later,the activities were rebounded but still lower than the control group (P<0.05).â‘¡During the continue training, at 4weeks,compared with the control group,the urine protein, urease, the activities of Na~+,K~+-ATP ase in kidney of the military-training group were increased obviously (P<0.05).At 8 weeks,the values of urine were continuely increased,whlie the activities of Na~+,K~+-ATP ase were decreased,and lower than the control group(P<0.05).Conclusions One-time exhausting military training decrease the activities of Na~+,K~+-ATP ase, continue moderate intensity military training can increase those activities,and continue high intensity military training can increase those activities.The last activities inverse correlated with the urease. The activities of Na~+,K~+-ATP ase in kidney directly influence the function of the renal tubule,and indirectly lead to hematuria and proteinuria.Second The changes of angiotensinâ…¡and AT1R in kidney during high intensity military trainingObjective To study the changes of angiotensinâ…¡and AT1R in kidney during the high intensity military training,to investigate the pathogenesis. Methodsâ‘ One-time exhausting training:Twenty four male SD rats,12 weeks old, were randomized into control group(n=8), military-training group(n=16)with the latter taking on one-time exhausting running exercises on treadmill according to the animal model. Observe the different values of urine , the concentration of angiotensinâ…¡in serum and kidney, the expressionin of AT1R in kidney by immunohistochemistry,right after training and 24 hours later.â‘¡Continue training:Forty male SD rats,4 weeks old, were randomized into control group(n=20)and military-training group(n=20)with the latter taking on continue moderate to high intensity running exercises on treadmill according to the animal model for 8 weeks. Observe the same values at 4 weeks and 8 weeks.Resultsâ‘ During the one-time exhausting training,compared with the control group,the urine protein, urease, the concentration of angiotensinâ…¡in serum and kidney, and the expressionin of AT1R of the military-training group were increased obviously right after training (P<0.05).24 hours later,the values were decreased but still higher than the control group (P<0.05).â‘¡During the continue training, at 4weeks,compared with the control group, the urine protein, urease, the concentration of angiotensinâ…¡in serum and kidney of the military-training group were increased obviously (P<0.05),while the expressionin of AT1R decreased obviously (P<0.05).At 8 weeks,all the values were higher than the control group(P<0.05).Conclusions High intensity military training can activate RAS in serum and kidney, and positive correlate with the urinary albumin. With the prolongation of the training time, RAS in kidney become more and more important.One-time exhausting military training activate RAS and increase the expressionin of AT1R.Continue moderate intensity military training activate RAS but decrease the expressionin of AT1R.,and continue high intensity military training can increase the expressionin.Partâ…¢The research of the preservation and healing about renal injury caused by high intensity military trainingObjective To study the respective protection of Losartan, Vitamin E, and traditional chinese drug during the renal injury caused by high intensity military training. Methods Fifty male SD rats,4 weeks old, were randomized into control group(C,n=10),military-training group(M,n=10), Losartan group(L,n=10), Vitamin E group(E,n=10), traditional chinese drug group(Z,n=10),with the latter four groups taking on continue moderate to high intensity running exercises on treadmill according to the animal model for 8 weeks. And enforce intragastric administration respective with Losartan 20mg/kg, Vitamin E 50mg/kg, traditional chinese drug 2ml in the latter three groups. Observe the different urine values every weeks,and the values in serum and urine at last.Results The urine protein became lower than M group at 3 weeks in L group,5 weeks in E group,8 weeks in Z group,but all the values were higher than C group(P<0.05).The values of urine protein at last were C