| Objective: To investigate the expressions of hTERT and Bcl-2 in cervical squamous cell carcinoma and effect of oridonin on human cervical cancer SiHa cell.Methods: The expressions of hTERT and Bcl-2 were examined in 89 cervical squamous cell carcinoma and 20 normal cervical specimens using immunohistochem- ical technique. The growth inhibition rates of SiHa cell were measured by MTT assay. Morphological changes of SiHa cell treated by oridonin were determined by Wright- Giemasa staining, Hoechst 33258 staining and transmission electron microscope (TEM). The apoptosis ratio of SiHa cell was defected by flow cytometric analysis (FCM). The expressions of c-myc, Bcl-2 and hTERT mRNA were determined by RT-PCR analysis.Results: In normal cervical specimens and cervical squamous cell carcinoma, the positive rates of hTERT were 20%, 76.4% respectively(P<0.01), and the positive rates of Bcl-2 were 35%, 79.8% respectively(P<0.01). The overexpression of hTERT was positively correlated with that of Bcl-2 (P<0.01,r=0.332). The proliferation of SiHa cells was significantly inhibited by oridonin in a dose and time dependent manner. Cell apoptosis ratio of 15μM Ori was 14.0% and the cell cycle was arrested at synthesis phase, but the apoptosis ratio of 30μM Ori decreased. Morphological changes of apoptosis of SiHa cell were observed by Wright-Giemasa, Hoechst staining and TEM. The expressions of c-myc, hTERT and Bcl-2 mRNA decreased by RT-PCR.Conclusion: hTERT and Bcl-2 overexpressed in cervical squamous cell carcinoma with promise well as a diagnostic target, both of them participated and promoted the tumorigenesis. Ori inhibited the proliferation of SiHa cells. Low concentration Ori induced Siha cells apoptosis, and high doze Ori led to apoptosis and necrosis. Apoptosis induced by Ori might be associated with inhibiting the expressions of hTERT,Bcl-2 and c-myc mRNA in SiHa cell. |
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