A Study On HIF-1α In Patients With Severe Acute Pancreatitis

Objective: Hypoxia-inducible factor-1(HIF-1) is an important transcription factor when mammal's organisms are in hypoxia condition. HIF-1 is a key regulator to maintain O2 homeostasis for whole body and cells, and it is also takes part in regulation of inflammatory response. Severe acute pancreatitis is a disease with severe inflammatory response and hypoxia within local and whole body, so to study the regulation effect of HIF-1αin SAP pathogenesis and clinical prevention and treatment will be significant valuable. Our study is to detected the expression of HIF-1αmRNA in white blood cells from patients with SAP by using RT-PCR, and the same white blood cells from healthy blood donators also tested as control. We also have been observed difference expression of HIF-1αmRNA in SAP and control, and trying to find the relationship between them, and that will provide a useful basis for the further research and helpful for clinical prevention and treatment in patients with SAP.Methods: Twenty patients with severe acute pancreatitis were admitted into ICU or Department of Digestion of the Second Affiliated hospital of Chongqing Medical University from March 2007 to September 2007 indued experimental group according the SAP criteria ,another twenty healthy blood donators as the control group at the same time. The white blood cells from the peripheral blood were isolated individually and measured the accounts using the isolation kit, then the total RNA were extracted by using the extraction kit. The expression and changes of HIF-1αwere detected by using RT-PCR, and the products were analyzed by using electrophoresis.Results: The electrophoresis of PCR product showed that HIF-1αmRNA expressed in most of the patients with SAP (fifteen of 20,75%), contrast to the control that just three of 20 donators (15%), this result indicated that the expression of HIF-1αwere significant different between patients with SAP and healthy blood donators.Conclusions: HIF-1a mRNA expressed in white blood cells from most of the patients with severe acute pancreatitis, it is implied that HIF-1 may take part in the pathogenesis of SAP.