Immune Effect After The Cryosurgery For The Mouse Bearing Liver Cancer

ObjectiveThe purpose of this study was to analyze the effect of Argon-Helium Cryosurgery(AHCS)on the cryoimmunologic responses of mice bearing H22 liver cancer through detecting splenocyte cytotoxicity,and expression pattern of cytokines T helper type 1(Th1)/Th2.Materials and MethodsSixty Balb/c mice were randomized into four group:the AHCS therapy group (group A,n=18),AHCS combined with Freund adjuvant group(group B,n=18),the tumor-bearing control group(group C,n=18)and normal control group(group D, n=6).Peripheral blood samples and the splenocyte of six mice were obtained from group A,B,C at 1w,2w,3w after the treatment,Peripheral blood samples and the splenocyte were obtained from group D before the treatment.The concentrations of interferon-γ(IFN-γ)and interleukin-4(IL-4),were detected by enzyme-linked immunoabsorbent assay(ELISA),the splenocyte cytotoxicity were detected by lactate dehydrogenase(LDH).ResultsH22 liver cancer cell has stable biology characteristics,and th e incadence of cancer was 100%.IFN-γconcentration were lower than that in normal group in three weeks(P＜0.05),but IL-4 was higher than that in normal group in two weeks in tumor-bearing groups(P＜0.05).It was higher than that in tumor-beating group for IFN-γconcentration in one week,two weeks and three weeks after AHCS therapy group,respectively(P＜0.05),but it was no significant differences between the AHCS and that of normal group in one week,two weeks and three weeks.It was lower than that in normal group and that in tumor-bearing group for IL-4 concentration in one week,two weeks and three weeks after AHCS therapy group(P＜0.05).It was higher than that in normal group and that in tumor-bearing group for IFN-γconcentration in one week,two weeks and three weeks after AHCS combined with Freund adjuvant group,respectively(P＜0.05),but it was no significant differences between that of the two and three weeks.It was lower than that in normal group and that in tumor-bearing group for IL-4 concentration in one week,two weeks and three weeks after AHCS combined with Freund adjuvant group(P＜0.05).Remarkably,IFN-γconcentration was higher in the AHCS combined with Freund adjuvant group than that in AHCS therapy group,but it has signify cant differences only in three weeks after the AHCS(P＜0.05).IL -4 was lower in the AHCS combined with Freund adjuvant group than that in AHCS therapy group,but it has signify cant differences only in three weeks after the AHCS(P＜0.05) Splenocyte cytotoxicity increased in the one week significantly,but decreesed in the two and three weeks in the group A,B and C.In the tumor-bearing group, splenocyte cytotoxicity in three weeks was lower than that in normal group and in one and two weeks(P＜0.05).In AHCS therapy group,splenocyte cytotoxicity in one and two weeks were higher than that in normal group and tumor-bearing group(P＜0.05).In the AHCS combined with Freund adjuvant group,splenocyte cytotoxicity in one and two weeks were higher than that in AHCS group and in normal group and tumor-bearing group(P＜0.05).ConclusionCryosurgery can effectively treat mice with H22 liver cancer,enhance splenocyte cytotoxicity to tumor cells,facilitate the secretion of Th1 type cytokines in mice with H22 liver tumors,promote the transformation from Th2 to Th1.Finally, Cryosurgery may increase the immune function of anti-tumor in tumor-bearing mice.The Freund adjuvant could enhandes this effect furtherly.