| Thalidomide may have been used the treatment of certain tumors in with their anti-angiogenesis effect. The purpose of this article is study the anti-tumor effect and mechanism with the combination of chemotherapy drugs through the concept of nude mice MHCC97-H cultivation in situ growth of the impact of thalidomide.Part1: the experimental animal models of ConstructionObjective: To establish experimental animal model for the further study of thalidomide in the mechanism of liver cancer and provide experimental platform. Methods: The first liver cancer tumor cell lines MHCC97-H after resuscitation training to the needs of the 2×107/mL cells, the tumor cells in nude mice inoculated to the right foreleg armpit subcutaneous tumor, when tumor's diameter became about 1cm sides, under aseptic conditions we will cut tumoe into pieces 1 mm3 of the small, in-situ in nude mice transplanted to the liver left lobe of the liver capsule. By weighing the nude mice inoculated in situ after the weekly weight to observe the changes in their living habits and finally the formation of the tumor in situ observations to study the effect of a tumor. Results: The success rate: heterotopic transplantation of success was 100% (5 / 5); orthotopic implantation model of success was100% (25 / 25). All the experimental nude mice after both survived to the end of the experiment, the survival rate of 100%. Conclusion: The Construction of the experimental animal models of innovative design approach, with a good rate of the original tumor cells consistent with the biological characteristics of the experimental liver cancer research a good experiment platform.Part2: the drug-bearing nude mice and the role of the determination of relevant indicators Objective: Through the in situ tumor in nude mice drug intervention, thalidomide in research on human hepatocellular carcinoma (HCC) angiogenesis and tumor growth invasive role of thalidomide in the mechanism of the HCC, as well as 5-FU in the HCC in the joint application. Methods: 25 in-situ bearing nude mice were divided into five groups, Group A: thalidomide in group 1 (administration after surgery first day), Group B: thalidomide in group 2 (administration after surgery fifth days), group C: 5 - FU group, the Joint Group D: (thalidomide in +5- FU) and the control group, Group E: (distilled water). Thalidomide in a way by gavage 200 mg / Kg / d administration, and 35 d; for 5-FU used by intraperitoneal injection of 20 mg / Kg / d administration for 5 d, afterward free drink distilled water. Section 35 d after delinking the mice were killed by cervical, removed mice's lung and tumor specimens, measuring the size of the tumor, the calculation of its size, weight, respectively, by immunohistochemical method (SP) of VEGF and microvascular density (MVD), with RT-PCR detection of VEGF and MMP-2 expression in the tumor tissue changes. All data had been analysised by SPSS13.0 statistical software. Results: Group D tumor size and weight significantly less than in group E and group A, group B, group C ,also has statistical significance. Group A, Group B and Group D of the block of VEGF expression and MVD and lung metastasis values and Group E more obvious statistical significance, and C groups also have significant difference. in tumor tissue five groups of MMP-2 expression no statistical difference. Conclusion: thalidomide as a kind of anti-angiogenesis agent can inhibit tumor growth and reduce lung metastasis. Nude mice bearing orthotopic liver cancer may be the main mechanism of tumor tissue by inhibiting the expression of VEGF and the formation of MVD to achieve, and for the expression of MMP-2 uncertainty. Furthermore, thalidomide in the united cytotoxic drugs 5 - FU for the treatment of HCC can produce synergy. |
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