The Experimental Study Of The Relation Between NSCLC, Combined With Cisplatin Chemotherapy And Vascular Endothelial Cell Damage

Purpose①To investigate the relationship between course of disease and venous endothelial cell (EC) damage in the non small cell lung cancer (NSCLC);②To investigate the effect on venous EC. administrating chemotherapy with cisplatin by intravenous injection (i.v.) regimen and intraperitoneal injection (i.p.) regimen in NSCLC mouse;③To investigate the relation between elevation of Von Willebrand Factor and activation of venous EC.Material and Methods 1)100 male mice were divided into control group and test group following randomization principle, 75 in the test group and 25 in the control group; 2 )The Lewis lung cancer model was made in test group; 3 )75 mice of control group were subdivided into three groups, 25 carcinoma natural progression group, 25 in cisplatin chemotherapy i.v. group and 25 in cisplatin chemotherapy i.p. group; 4) Specimen was get in 3 days, 7 days, 14 days , 21 days and 28 days after cispletin chemotherapy. The morphology and ultramicrostructure of venous EC, the adhesion of vWF in venous intima were observed.Results 1)By electron microscope, venous EC damage developed over time in all three groups. Venous EC in static state was activated and showed hypertrophy of cell body with rough surface, profuse secrete granule in the cytoplasma, thickening Weibel—Palade body and several ECs detached from base membrane; 2) The amount of vWF staining granules in all three groups gradually increase in 7 days, 14 days and 21 days after cisplatin chemotherapy; At the same time (7 days, 14 days , 21 days and 28 days) point, there was no statistic difference for the amount of the aggregate vWF staining granule in the intima between i.v. cisplatin group and i.p. cisplatin group . Compared with carcinoma natural progression group, the amount of the aggregate vWF staining granule are larger in the two cisplatin groups, with statistic difference.Conclusion 1)Venous EC damage of the Lewis mice increased over time; 2)Cisplatin chemotherapy can aggravate venous EC damage and EC damage level had no relevant to i.v. or i.p. regimen; 3)Increased level of vWF in Lewis mice may related to the activation of venous EC by cancer cell.