The Study Of Relation Between Gene Polymorphism Of Vascular Endothelial Growth Factor- 2578C/A And Non-small Cell Lung Cancer

Objective: Through exploring the correlation between polymorphism loci of vascular endothelial growth factor(VEGF)-2578C/A gene and nonsmall cell lung cancer(NSCLC), to discuss the correlation between single nucleotide polymorphisms(SNPs) of-2578A/C in promoter region of VEGF gene and the genetic susceptibility to NSCLC, so as to further clarify the molecular biological mechanism of NSCLC and provide theoretical basis for prevention and treatment of NSCLC.Methods: A case-control study was carried out in this paper. Venous blood was taken from 111 lung cancer patients and 89 healthy controls.Medical history and personal details of each case were recorded. DNA was extracted from peripheral venous blood specimen and was sealed and preserved in a refrigerator at-20 ℃. Ligase detection reaction-polymerase chain reaction(LDR-PCR) was used to analyze the genotype of-2578A/C in promoter region of VEGF gene and the alleles.SPSS 19.0 was used for statistical analysis, with P<0.05 as of statistical significance. Chi-square analysis was used to analyze the differences of two groups in distribution of genotypes and frequency of alleles. Non-conditional Logistic regression was used to calculate the ratio of relative risk degree(odds ratio, OR) and 95% confidence interval(CI). P<0.05 referred to statistical differences. The two groups were compared for frequency of genotype, allele frequency, OR and 95% CI. And the correlation between polymorphism loci of VEGF-2578C/A gene and the susceptibility to NSCLC was analyzed.Result:1 Distribution frequency of genotype(C/C, C/A, A/A) were 46.1%, 2.2%and 51.7% in the healthy control group and 62.2%, 9.9% and 27.9% in thelung cancer group, with statistically significant differences(P=0.001, X2=14.030, OR=2.497, 95% CI of 0.295-0.916). Since the lower frequency of genotype A/A, genotype C/A and genotype A/A were merged. Then the frequency of genotype C/C and genotype C/A+A/A were 46.1 and 53.9% in the healthy control group, while 62.2% and 37.8% in the lung cancer group.There were statistically significant differences between two groups in distribution of genotypes(P=0.0023, X2=5.170, OR=0.520, 95% CI of 0.295-0.916). Compared with genotype C/A, genotype C/C could increase the risk of lung cancer.2 Frequency of allele C and allele A of-2578C/A SNP in promoter region of VEGF were 76.1% and 23.9% in the NSCLC group, while 68.1%and 31.9% in the healthy control group, with statistically significant differences(P<0.05). There were significant differences between allele C and allele A(X2=3.297, P=0.044). Compared with allele A carrier, allele C carrier could increase the risk of NSCLC.Conclusion:1 Polymorphism loci of VEGF-2578C/A gene may increase the risk of NSCLC.2 Compared with genotype CA, genotype CC may increase the risk of NSCLC. And gene C is the risk factor of NSCLC, namely allele C carrier may increase the risk of NSCLC.