A Study On The Correlation Between The Polymorphism Of Human Leucocyte Antigen-DRB1 Alleles And Familial Hepatitis B

Objective 1. To understand clinical epidemiology features of familial hepatitis B by investigating the familial hepatitis B families in Shanxi. 2.To study the correlation between HLA-DRB1 alleles and familial hepatitis B.Methods 1. All the subjects were detected in liver function by routine biochemistry, HBV serologic marker by ELISA, the serum HBVDNA load by PCR and abdomen check by colorful B supersound. The collected datum were analyzed from four aspects as follows:â‘ Whether there are some differences in the infection rate between children from HBsAg-positive mother and those from HBsAg-positive father.â‘¡Whether there are some differences in the HBV infection rate between the children from the countryside and those from the urban.â‘¢Whether there exists a relationship between the HBV infection rate and sex.â‘£Whether there are different outcomes of between children from HBsAg-positive father and those from HBsAg-positive mother.2.The HLA-DRB1 alleles of 151 people were detected in the familial hepatitis B families, by using the Polymerase Chain Reaction-Sequence Specific Oligonucleotide probing(PCR-SSOP)technique and then a statistical analysis is conducted in the experimental results .Results 1.The probability of children infected by HBsAg-positive mother is higher than that of HBsAg-positive father (P<0.05); The HBV infection rate of children in the countryside is higher than in the urban(P<0.05); The HBV infection rate is not significant in sex in the familial hepatitis B families; There are no significant differences in the outcome between children infected by HBsAg-positive father and by HBsAg-positive mother. 2.The allele frequency of HLA-DRB1*0701 in the chronic hepatitis B(CHB) group was markedly higher than that in the normal control group(17.8%vs7.4%),with significant correlation between them(P<0.05, OR=2.725). The allele frequencies of HLA-DRB1*0401/0403/0405 and HLA-DRB1*1301/1302 were markedly lower in the CHB group than that in the normal control group(5.1%vs16.2%; 0%vs4.4%),with statistical significance (P<0.05,OR=0.278;P<0.05). There are no more significant differences in all of HLA-DRB1 alleles between the chronic HBsAg carrier group and the CHB group, the chronic HBsAg carrier group and the normal control group.Conclusion 1.Mother-infant transmission is the main route; There are more children infected by HBV in the familial hepatitis B families in the countryside than in the urban; The HBV infection rate of children in the familial hepatitis B families is not linked to sex; There are no obvious differences in the outcome between the children infected by HBsAg-positive father and those by HBsAg-positive mother. 2.HLA-DRB1*0701 is closely relevant to the susceptibility to familial chronic hepatitis B,and may be the susceptible or linkage gene. HLA-DRB1*0401/0403/0405 and HLA-DRB1*1301/1302 is closely associated with the resistance to familial chronic hepatitis B, and may be the resistant gene. These findings offer an implication that host HLA-DRB1 is an important factor predicting the outcome of familial HBV infection.