| Objective:to investigate changes in Th1Th2cytokine,oxidation and antioxidant ingredients in different pathological durations,then infer immune mechanism,role of oxidation and anti-oxidative damage to the Eales disease in the process and evaluateMathod:1.Sample selection and processing:30 Eales disease patients,first diagnosed in the Department of ophthalmology,The PLA General Hospital,all were male.Minimum age of 18-year-old,maximum age 45,The average age of 29.29±8.67 years,follow-up time for 3-15 years,an average of 7.1 years.Among that patients,12 were in NVH period group vs 18 VH patients.The age of 19-41 years old,The average age of 32.5±4.53;VH group of 20 patients,age of onset was 18-45 years old,an average of 26.5±12.53.The control group was 10 cases,all the hospital staff.They all were males,aged between 18 an 45-year-old,no autoimmune diseases and family history of genetic disease,unrelated, normal body and eye examination;Corticosteroid therapy group:a total of 16 cases,male. Minimum age of 24-year-old,maximum age of 38-year-old,The average age of 27.29±4.67 years follow-up time is 3-15 years,with an average of 6.9 years 7:00 am all selected cases were taken blood from The elbow about 4 ml at quiet,fasting state.After centrifugal (4℃,3000 rpm,10min)1 hour,sucked with micro-tips from the upper serum of blood about 0.5 ml,points installed in Eppendorf tube,numbered,-70℃saved standby to prohibit The repeated freezing and Thawing.2.The subject used liquid-chip analysis system (luminex100TM),combining traditional ELISA,immunohistochemistry and fluorescence to detect The cytokines(IL-2,4,5,10,TNF-α,IFN-γ)level in different Eales disease duration.we compared the contents of cytokines in the serum of patients with Eales and its impact on Eales disease,analyse Eales disease' risk factors and reduce the incidence of Eales disease and to find a better way to prevent and treat the disease.3.Use of SOD,NO kit to analyze the serum content of SOD,NO in The patients and the impact of oxidation, anti-oxidation imbalance of the of Eales disease4.Statistical analysis:T test used to analysis of related risk factors,logistic regression model with multi-factor analysis of risk factors could leading to Eales disease and the risk of size. Result:1.HIFN-γ:the results of the statistical analysis showed that in The inflammation duration,the level of serum HIFN-γwas no significant difference compared with control group(P≥0.05);VH group was significantly higher than control group(P＜0.05);glucocorticosteroid group was also significantly higher than control group(P＜0.05);NVH group and VH group was no significant difference(P≥0.05);NVH group, VH group and glucocorticosteroid cured group were no significant difference(P≥0.05). 2.IL-10:the level of serum IL-10 in NVH group was no significant difference competed with control group(P≥0.05);VH group was significantly higher than control group(P＜0.05);glucocorticosteroid group was significantly higher than the control group(P＜0.05); VH group was significantly higher than NVH group(P＜0.05);NVH Eales group and glucocorticosteroid group were no significant difference(P≥0.05);group of VH was significantly higher than glucocorticosteroid group(P＜0.05).3.IL-2:the blood level of IL-2 patients was no significant change in different periods compared with the control group(P≥0.05).IL-2 did not significant change with the development of disease.4.IL-5: The results of the statistical analysis showed that the NVH phase Eales patient's serum IL-5 in the control group and no significant difference(P≥0.05);group of VH, glucocorticosteroid cure the patient's group Eales serum IL-5 levels were significantly higher than the normal control group(P＜0.05);VH phase Eales patient's serum IL-5 was significantly higher than the NVH phase group(P＜0.05);NVH phase group,VH group of the patient's serum IL-5 levels were significantly higher than the glucocorticosteroid group (P＜0.05).5.IL-4:the level of serum IL-4 in NVH group was significant lower than the control group(P＜0.05);group of VH was significantly higher than the normal control group(P＜0.05);glucocorticosteroid group was no significant difference with the control group(P≥0.05);VH group was significantly lower than the glucocorticosteroid group (P＜0.05).6.TNF-α:The results of the statistical analysis showed that the serum TNF-αlevels in NVH group,VH group were significantly higher than the control group(P＜0.05); glucocorticosteroid group was no significant difference compared with the control group(P≥0.05);NVH group was significantly higher than VH group(P≥0.05);NVH group were higher than glucocorticosteroid group(P＜0.05).7.SOD results of the statistical analysis showed that level of serum SOD in different periods Eales of patients were significantly different with that the control group(P＜0.05);NVH group was significantly higher than VH group(P＜0.05);NVH group,VH group were significantly lower than glucocorticosteroid group(P＜0.05).8.NO results of the statistical analysis showed that level of serum NO in different periods Eales of patients were significantly different with that in the control group(P＜0.05);after glucocorticosteroid therapy serum content of NO was no significant difference with the control group(P＞0.05).VH and NVH groups were not significant difference(P＞0.05);NVH group,VH group were significantly lower than the glucocorticosteroid group(P＜0.05).Conclusions:1:Th cell subsets plays an important role in Eales disease'development 2:Reducing Th1-produced cytokines and balance the Th1/Th2 T-lymphocytes may possibly treat the Eales disease.3.Glucocorticoid treatment of Eales disease especially in NVH group take a significant effect,but some cytokines are still on a high level,suggesting that there is still a possibility recurrence of Eales disease.4.Oxidative stress is also closely related to Eales disease;free radicals elimination may also play an important role in The treatment of Eales disease. |
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