Associated Study About The Effect Of Helicobacter Pylori Infection On MMP-2 Expression In Different Gastric Mucosa Lesions

OBJECTIVE: To detect the expression of MMP-2 and H. pylori state indifferent gastric mucosa lesions, that is general population, mild, modestand severe dysplasia patients and gastric cancer patients. To study theeffect of H. pylori infection on MMP-2 expression in different gastricmucosa lesions and on the invasion and metastasis of gastric cancer.METHOD: A total of 293 cases were available for the study including52 cases of chronic superficial gastritis to serve as control group, 71cases of mild dysplasia, 74 cases of modest and severe dysplasia and 96cases of gastric carcinoma; H. pylori was determined with Giemsa stainingmethod, rapid urease test and ¹⁴C urease breathtest; Streptavidin-peroxidase (SP) immunohistochemical method was used todetect the expression of MMP-2 in different gastric mucosa lesions forthe purpose of analyzing the effect of n. pylori infection on MMP-2expression.RESULTS: No MMP-2 was expressed in control group. The positive rateof MMP-2 expression in mild, modest and severe dysplasia and gastriccarcinoma were 49.3ï¼…, 70.3ï¼…and 87.5ï¼…respectively. The positive rate ofMMP-2 expression in gastric carcinoma was significantly higher than thatin chronic superficial gastritis (P＜0.01) and that in mild, modest andsevere dysplasia (P＜0.01). In modest and severe dysplasia the positiverate of MMP-2 expression was significantly higher than that in milddysplasia (P＜0.01) as well as in control group (P＜0.01). In mild, modestand severe dysplasia and gastric carcinoma the positive rate of MMP-2expression with H. pylori infection(61.5ï¼…, 80.5ï¼…, 94.8ï¼…) weresignificantly higher than that without H. pylori infection (34.4ï¼…, 57.6ï¼…, 76.3ï¼…) respectively(P＜0.05).The incidence of lymph node metastasis ingastric carcinoma with H.pylori infection (74.1ï¼…) was significantly higher than that without H. pylori infection (36.8ï¼…) (P＜0.01); Thepercentage of TNMⅢⅣpatients in gastric carcinoma with H. pyloriinfection (79.3ï¼…) was significantly higher than that without H. pyloriinfection(50ï¼…)(P＜0.01). The positive rate of MMP-2 expression in gastriccarcinoma with lymph node metastasis (98.2ï¼…) was significantly higherthan that without lymph node metastasis (71.8ï¼…) (P＜0.01); The positiverate of MMP-2 expression in gastric carcinoma of TNMⅢⅣstage (100ï¼…)was significantly higher than that of TNMâ… â…¡stage patients (61.3ï¼…)(P＜0.01).CONCLUSION: H. pylori infection can stimulate the MMP-2 expression indifferent gastric mucosa lesions, that is mild, modest and severedysplasia and gastric carcinoma. The positive rate of MMP-2 expressionin gastric carcinoma was significantly higher than that in control groupand that in mild, modest and severe dysplasia. H. pytori infection playsimportant roles in the development as well as the invasion and metastasisof gastric carcinoma. The eradication of H. pylori is very important.